We narrowed to 672 results for: FIE

1. Plasmids 101: Methylation and Restriction Enzymes

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   ... a pool of mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme...

2. How Addgene Creates Content

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   ...resources with our antibody and content team, we identified what applications should be included and what...

3. AAV Q&A with Tim Miles

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   ...allow AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms...

4. Antibodies 101: ChIP

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   ...analysis via any methods suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s...

5. Sweating the Small Stuff: Details in the Lab

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   ...to Try  Checklists Found in surgical suites, air fields, and military bases, checklists are the ultimate...

6. Antibodies 101: Buffers, Storage, and Conjugates

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   ...introduction to the wide, wild, and fascinating field of signaling molecules. Assay Type Westerns and ...

7. Antibodies 101: Monoclonal Antibodies

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   ...produced by the hybridoma. Any genetic drive can be identified by sequencing the heavy and light chains. To ...

8. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...and meet our QC standards. If any concerns are identified, we can quickly put a hold on all orders and ...

9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either a 1-7 base poly(U)tail in...

10. CRISPR 101: Non-Homologous End Joining

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    ...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...

11. How to Make Friends and Meet People at a Scientific Conference

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    ...scientists they know personally Scientists in your field will know about grants and projects that might help...

12. Transferable Skills Guide: Public Speaking

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    ...native language. And, at least in the biomedical field, with very little text on the slides and notes instead...

13. The time and cost required to make a plasmid

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    ...people doing interesting and relevant work in your field! However, there’s no guarantee you’ll get a response...

14. Celebrating 15 Years of Scientific Sharing

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    ...collaboration and innovation between legal and scientific fields.” Thanks to our legal team, and great customer ...

15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ...proteasome degradation. Autophagy is then induced and quantified by measuring the amount of radioactivity in the...

16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ... insert of interest. Hence correct clones are identified much more efficiently, as those that do not contain...

17. Developing Lab Management Software for Biology

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    ... your lab bench, we need to (this is a little simplified): Find the appropriate glycerol stock within...

18. Antibodies 101: Conjugation

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    ...commercially available conjugated antibody will have been verified to perform the same as its unconjugated version...

19. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...836-42. PubMed PMID: 28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J....

20. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    ...expression and plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging from 3-100...