Addgene

We narrowed to 665 results for: FIE

Showing: 521 - 540 of 665 results

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GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...that wasn’t enough, in this development-focused field, it’s not uncommon to find protocols that require...
How Addgene Creates Content

Type

Blog Post

...resources with our antibody and content team, we identified what applications should be included and what...
Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

... a pool of mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either a 1-7 base poly(U)tail in...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...
How to Make Friends and Meet People at a Scientific Conference

Type

Blog Post

...scientists they know personally Scientists in your field will know about grants and projects that might help...
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

Type

Protocol

...Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose...
Antibodies 101: Affinity Reagents

Type

Blog Post

...situations where a suitable antibody has been identified but a smaller or less immunogenic version is ...
Transferable Skills Guide: Problem Solving

Type

Blog Post

...resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset...
Antibodies 101: Polyclonal Antibodies

Type

Blog Post

...recognizing your protein of interest: affinity-purified antibodies are isolated from the serum by their...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

...to form a protein identical in sequence to the unmodified enzyme. Each half of the base editor is fused...
Celebrating 15 Years of Scientific Sharing

Type

Blog Post

...collaboration and innovation between legal and scientific fields.” Thanks to our legal team, and great customer ...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...expression and plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging from 3-100...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...proteasome degradation. Autophagy is then induced and quantified by measuring the amount of radioactivity in the...
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

... insert of interest. Hence correct clones are identified much more efficiently, as those that do not contain...
Transferable Skills Guide: Public Speaking

Type

Blog Post

...native language. And, at least in the biomedical field, with very little text on the slides and notes instead...
The time and cost required to make a plasmid

Type

Blog Post

...people doing interesting and relevant work in your field! However, there’s no guarantee you’ll get a response...
Antibodies 101: Conjugation

Type

Blog Post

...commercially available conjugated antibody will have been verified to perform the same as its unconjugated version...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

...836-42. PubMed PMID: 28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J....
CRISPR 101: Cas9 vs. The Other Cas(s)

Type

Blog Post

... Cas12a – The staggered cutter Originally classified as Cpf1, Cas12’s primary difference from Cas9...

Showing: 521 - 540 of 665 results

Results per page:

GCE4All: Making Genetic Code Expansion Accessible

How Addgene Creates Content

Plasmids 101: Methylation and Restriction Enzymes

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CRISPR 101: Non-Homologous End Joining

How to Make Friends and Meet People at a Scientific Conference

Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

Antibodies 101: Affinity Reagents

Transferable Skills Guide: Problem Solving

Antibodies 101: Polyclonal Antibodies

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Celebrating 15 Years of Scientific Sharing

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Transferable Skills Guide: Public Speaking

The time and cost required to make a plasmid

Antibodies 101: Conjugation

Lighting Up Cell Signaling with Photoswitchable Kinases

CRISPR 101: Cas9 vs. The Other Cas(s)

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