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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 521 - 540 of 856 results

  3. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...light of a different color instead. This approach has even been used in biosensors where BRET reports on...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Singer’s lab at Albert Einstein College of Medicine has recently published techniques to follow translation...Rojas! Eugenia Rojas, PhD is a neuroscientist who has always been deeply interested in neurobiology.  She...

  6. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ... human suffering and death. Doing research right has the potential to save lives and money. A 2014 analysis...physiologic or pathologic processes. Much progress has been made in this area in recent years, and you can...

  7. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor...allosteric inhibitors at Novartis. The ClonTracer library has been distributed all over the world and was recently...

  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...ends interfere with that step. Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases...

  11. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...base editing to pooled library screening. It also has information for planning your CRISPR experiment. ...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits focused on optimizing prime...collaboration with the Vallabh/Minikel Lab, the Weissman Lab has developed a new epigenetic editor, Coupled Histone...subtypes, and a familiar challenge for anyone who has done histology on brain tissue is identifying the...

  13. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...served by a one-sample test as the standard value has no variation that needs to be accounted for. ANOVA...multiple range test, and several others. Each test has different likelihoods of returning false positives...to a single group. Dunnett’s is more powerful and has a lower error rate than the multiple comparison tests...

  14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...including creating Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method...left homology arm guanine (i.e. GG); this design has been shown to increase T7 promoter activity. To avoid...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...well if you’ve been following the Addgene blog, it has been repurposed for genome editing in eukaryotes,...editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (Zhang et al., 2016...than that, be nice to your microinjectionist, who has the strategically important job of delivering the...

  16. Deep Dive: qPCR

    Type
    Blog Post
    ... a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while...for all amplicons; if your amplicon is AT-rich or has a secondary structure, you may see a double peak ...being a common choice - the frequently used GAPDH has noted issues as a reference gene (Higgins, 2000; ...assumptions that match your data set, that is.) Yuan, 2006 has a good overview of different statistical analysis...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...-homologue recombination was released. This blog has not been updated in response to this paper. Any hint... hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published...

  18. To Each HIS Own

    Type
    Blog Post
    ...can be pretty complicated given that a single cell has tens of thousands of proteins functioning in a variety... would not be able to do. We hope that this blog has answered all of your questions about polyhistidine...

  19. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...

  20. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...women. Bicuspid aortic valve (an aortic valve that has two leaflets, instead of three) and acute aortic ...intracellular signaling,” says Hébert. Previous research has shown that GNB1 mutations lead to increased activity...
Showing: 521 - 540 of 856 results