We narrowed to 851 results for: Met
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TypeBlog Post...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding...
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Our Most Popular Blog Posts from 2019
TypeBlog Post... scientific research. Get an overview of these methods in this blog post. Hope you’ve enjoyed going through...there any topics you particularly like or maybe something you’d like to see more of? Let us know by leaving... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post... in terms of contamination, but consider flow cytometers. These fluidic machines handle biological samples...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...while some might argue that the only way to do something is to do it huge, there are times when a more ... -
Editor's Choice, August 2016
TypeBlog Post...this month, I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...taking a look at all of our posts as each one has something great to offer, I just need to get back to emailing... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...an indication of the stiff competition or says something about my cooking. This is a creative (and delicious...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year... -
Science as Art
TypeBlog Post... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...new x-ray technique provides scientist with a new method to explore the inner structures of delicate plants... -
Celebrating Accomplishments in the Lab
TypeBlog Post... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post... for monitoring norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592...Making protein folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog Post...out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence...among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and eat, and...vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know in the ...Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering Interested in... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is...Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...peptides sequences into these variable loops is one method for changing a capsid’s tropism. cap also encodes...the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org... -
Fostering communication in remote collaborations
TypeBlog Post...scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog... -
Plasmids 101: What is a plasmid?
TypeBlog Post...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...the figures of interest along with the article metadata, legend and user created notes on a “Pinterest...Christa Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure.... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...fingerprint can be detected. Bourdeau et al calls this method acoustic collapse. Importantly, acoustic collapse...have already developed a high-throughput screening method for serial acoustic collapse to aid in the development... -
10 Ways to Share Your Science!
TypeBlog Post...ask and answer questions and hopefully discover something new. Science involves success and, more often,...not just the details of your science but also something about how science really works. 2) Read science... -
RUBY-Red Siliques
TypeBlog Post... instead, which can’t be done if your selection method kills the transgene free individual. For example...studying large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright...