...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

... vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound... experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration...

...for our quality control process because of its expansive feature library and useful tools. In this blog...proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization...

...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

...descriptions often fail to capture all of the responsibilities of a role, so it helps to see how people describe...path one you could see yourself taking? If so, consider setting up an informational interview with them...

...at his University of Wisconsin lab for a summer during my PhD and is responsible for my enduring love ...

...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

...for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric gRNA...screened? A4: Before starting your experiment, consider co-transfecting with GFP. This allows you to sort...

...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

...Addgene's online resources. Career Videos Get career insight and advice from real Addgenies! Protocols (Link... once using a multichannel pipette and avoid inconsistent pipetting with our simple tips Bacterial Transformations...Australia, and advice for current graduate students considering their future careers. Eric J. Perkins, PhD In...Addgene's Business Development Manager, discusses his transition from the Scientist Team to Business Team, his...

...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...

...Since the start of this crisis, we’ve been very responsive to the mood of the Addgenies — and they were ...unfortunately we believe it is best to further reduce onsite operations for the coming week. In an abundance...

...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...

...2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy...

...interpretations. A good peer-reviewer will help spot inconsistencies in the work, missing information that would...non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my sentences might sound...

...replacing its dimerization domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light... Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat Chem Biol....

...distributions to cover costs, which can limit expansion into new services. “We appreciate NIH’s vision.... The content of this article is solely the responsibility of the authors and does not necessarily represent...

...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial... cores and laboratories alike as an easy and inexpensive method to improve AAV yields. Although we only...