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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 882 results for: NSI

Showing: 521 - 540 of 882 results

  1. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... check to make sure that it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase...opens in a new window) NEB's table of methylation sensitive restriction sites . Sometimes enzymes cut sequences...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....

  3. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  4. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...cupcakes, or maybe the lab member originally responsible for the deposit had already moved on to their...with journal articles. But we do encourage you to consider sharing them openly as soon as that preprint is...

  5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

  7. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

  10. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

  11. Fostering communication in remote collaborations

    Type
    Blog Post
    ...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

  14. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

  15. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...

  16. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...

  20. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...
Showing: 521 - 540 of 882 results