Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 902 results for: Nes

Showing: 521 - 540 of 902 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ... Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic... as everything from parasites, to symbionts, to genes and the labels applied to them were vague or contradictory...biology was never the same. Over the next few years genes from different bacterial (and eventually mammalian...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...new method uses a selection cassette containing genes for hygromycin resistance and the Roller movement...testing, Dickinson et al. tested this system across 7 genes in parallel; when they injected 60-80 animals per...desired insertions on the first attempt for 6/7 genes. The data are clear - the SEC-based system is both...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...treat AD using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset ...diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice with a dominant...efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are still looking...

  6. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...different institutes, disciplines, and cultural backgrounds.” This work outlines the need for clear communication.../doi.org/10.1371/journal.pcbi.0030044 Wuchty S, Jones BF, Uzzi B (2007) The Increasing Dominance of Teams...

  7. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...potential human helpers of SARS-CoV-2 (especially the ones that are druggable) is a key step in our strategy...as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI restriction sites...study - so you might want to be careful with these clones (and help us figure out what is going on there!...

  8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...reporter that has a large dynamic range and high brightness. The Shu Lab from UCSF designed an improved apoptosis...reporter that has a large dynamic range and high brightness. This reporter achieves approximately 100-fold...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

  9. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...scientists and the public together to build consensus guidelines, but there was never a specific timeline for ... not remembered kindly. However, despite many headlines questioning if He’s experiments prove otherwise...genetics this time?” She lives with her partner (in business and life) in an old Girl Scout Cookie factory ...

  10. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ... on and off by binding proximal to their target genes. Since gene expression patterns determine everything...genetic and expression control systems to mimic illness on a molecular level. Atlas resource The TF map...from the library to further investigate specific genes and TF isoforms. Due to the size of the collection...

  11. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...directed transcription from a given promoter. To harness the power of light, Christopher Voigt’s lab created...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...is digested with either HindIII and BamHI alone (lanes 4-5), there is a single band of 7.3 kb representing...interpret the bands that you get in your sample lanes. Choose your ladder based on the expected band sizes...supercoiled, relaxed and nicked. If your digest lanes look like your uncut lane then there is something...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...IF-ICC worked well for identification of the correct clones, it was determined that for some constructs more...more than 90% of the colony PCR validated clones unexpectedly failed to produce functional R-mAbs. It was...were created. This greatly limited the number of clones that needed to be screened through functional assays...

  14. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...difference (Figure 3). If you are interested in harnessing the power of digital PCR screening to validate.... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...requirement, gRNA, and multiplex ability Experimental guidelines and applications Protein structure and properties... documents the target substrate (DNA/RNA and strandedness) along with its action on the substrate (nick...can I use it? CasPEDIA operates like many search engines, featuring both a general search bar tool and an...

  17. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...quantifiable readouts for levels of different chemicals, hormones, proteins, etc., that you are interested in. The...potentials; it’s an ion of interest for many disciplines! Most of the sensors designed for calcium generate...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...

  18. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson, 1982)....biological research, you might have an idea which ones could be helpful to you. Interested in learning ...Therapies in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001...

  19. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back on itself in a closed...that expressed membrane-localized mCherry. With darkness, SOS binds Ras and initiates the extracellular...These results demonstrate that OptoNBs can be harnessed to regulate signaling pathways such as the Ras...

  20. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...through my work with the renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts... 3 topics and write them on a board to see which ones get the most hits. Commit to a meeting schedule ... get to present formally often enough) and give honest, critical feedback for improvement. Go to a science...
Showing: 521 - 540 of 902 results