A Deep Dive into BioBuilder
Type
Blog Post
...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems.
Today...
We narrowed to 653 results for: PPL
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Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...a gene of interest is one of the most common applications of precision genome editing, the remainder of... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...in my own research. Again, some of this may not apply or may not work for you. The best way to find a ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall... -
Plasmids 101: Blue-white Screening
TypeBlog Post...certain media components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...analyzing the data. It should be straightforward to apply the new library for use in various cell types and... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ... -
dTAG - You're it!
TypeBlog Post...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...developed by the James and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...some reporters are better suited for particular applications. Read on to learn more about each reporter or... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...comes to public speaking, Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some... -
What's New in CRISPR - November 2021
TypeBlog Post...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease.... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...kinases good for? There are a number of potential applications for photoswitchable kinases. Below are a few...