We narrowed to 651 results for: PPL

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...

3. A Deep Dive into BioBuilder

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   Blog Post

   ...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...

4. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   ...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...

5. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...

6. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...in my own research. Again, some of this may not apply or may not work for you. The best way to find a ...

7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall...

9. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   ...certain media components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may ...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...

11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    ...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...

12. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...comes to public speaking, Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal...

13. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...

14. What's New in CRISPR - November 2021

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    Blog Post

    ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

15. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...

16. dTAG - You're it!

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    Blog Post

    ...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective...

17. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...developed by the James and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell...

18. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    ...some reporters are better suited for particular applications. Read on to learn more about each reporter or...

19. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...