We narrowed to 605 results for: SHE

...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions....

...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction...

...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

... Alyssa Shepard...

...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing...

...-58223-5.   TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search...

... Alyssa Shepard...

... Alyssa Shepard...

...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...

...vector. For example, although researchers know that Usher Syndrome (a disease that results in combined deafness...

...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...