Plasmids 101: Modular Cloning
Type
Blog Post
... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...
We narrowed to 609 results for: SHE
-
-
Tips for Titering Your Lentiviral Preps
TypeBlog Post... a project forward, transductions should not be rushed into. Before you start any transduction, you should... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post... Alyssa Shepard... -
Common Injection Routes in Mice
TypeBlog Post... Alyssa Shepard... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... Alyssa Shepard... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post... References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post... is generally advised, there is little detail published on how to do it best. Scientists are typically... -
Hot Plasmids: Summer 2025
TypeBlog Post...-58223-5. TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions.... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed... -
Genome-wide Screening Using CRISPR
TypeBlog Post...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance... -
Which Fluorescent Protein Should I Use?
TypeBlog Post... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine... -
An Introduction to Adenovirus
TypeBlog Post...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...