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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 618 results for: Set

Showing: 521 - 540 of 618 results

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...photoactivatable CRISPR gene activation system, but settled on a different optimal fusion protein combination... the protein’s ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect the CRISPR...pluripotent stem cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318–331...

  5. Antibody Guide

    Type
    Guide
    ...non-biological replicates) often each require their own set of controls. Multiplex Assays Flow cytometry, IF,... cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires...requires single-antibody controls, a small subset of the sample labeled with only one antibody, for each ...

  12. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ... chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer Lab’s decorated office...

  15. What's New in CRISPR - May 2019

    Type
    Blog Post
    ... All-in-one AAV delivery of Nme2Cas9 with gRNA cassette Erik Sontheimer’s lab identified a Cas9 protein...
Showing: 521 - 540 of 618 results