We narrowed to 716 results for: cas
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TypeBlog Post...designations all refer to the same test. On some occasions, there is an experimental group being compared... being between control vs. experimental. In this case, save yourself from family-wise error and just perform...
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Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...gene of interest with an antibiotic resistance cassette. Substrate DNA Design and Generation Whether...can be used to screen for positive clones in most cases, and restriction enzyme digest can be used to screen... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...antibodies together in the same color panel. In this case, make sure to compare the excitation/emission spectra... so it's better to use a brighter fluorophore in case the CD45 expression turns out to be weak. Table ... -
Deep Dive: qPCR
TypeBlog Post... double peak even with a single product. In that case, you’ll want to validate your primer/probe through...Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate choice (Panina, 2018... -
Delivery Methods for Generating iPSCs
TypeBlog Post... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...ability to cross the BBB, or the PNS, while in many cases targeting the liver less. Because these systemic...being geographically constrained to the brain. In cases where it’s critical to minimize or eliminate off-target... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...the video to find out the final four! (Note: For cases where the same name was submitted by more than one... -
Addgene's 20th Anniversary Party
TypeBlog Post...headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the party. There are so ... -
It’s CRISPR Week at Addgene!
TypeBlog Post...knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications... -
2021 Deck the Lab Winners!
TypeBlog Post... were absolutely abuzz with anticipation as they cast their ballots for their favorite submission. So ... -
Twenty Years of Sharing Science!
TypeBlog Post...it's hard to summarize, but we wanted to mark the occasion by trying. When we started in 2004, we were... -
The Codon Guide to Synthetic Biology
TypeBlog Post... blogs, free courses, YouTube channels, books, podcasts, and communities to help people get started in... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog Post...response GIF. We have GIFs for every occasion. Disaster in the lab you haven’t quite gotten ... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason... -
Call for Guest Bloggers
TypeBlog Post...editing. Post: Generating Mouse Models Using CRISPR/Cas9 Erik L. Snapp, Associate Professor at Albert Einstein... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog Post...Home Edition This edition of the contest should showcase a winter or holiday theme decor in your work from... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday: Why deposit... -
Countdown to Halloween @Addgene
TypeBlog Post...Halloween and how seriously we take costume design. In case you need more convincing, I’ve got just the proof... -
Addgene’s Blog Gets a New Look!
TypeBlog Post... the navigation menu. The new navigation menu showcases the five main topics of the blog: Plasmids CRISPR...