Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 704 results for: des.1

Showing: 521 - 540 of 704 results

  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... serotonin, and much more. Check them out! Table 1: New ready-to-use viral vectors in the Addgene repository...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...

  2. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...updated version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation...in the initiation of a vast number of signaling cascades, inducing different cellular responses. One of...localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate...protein signal transducers. The TRUPATH collection provides scientists with a toolkit to gain an understanding...

  3. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes ...et al., 2018). These sensors also exhibit high magnitudes of fluorescent responses. Studies on the kinetics...Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396...

  4. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...materials to the research community. References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure... to improve viral vector production. Whether redesigning viral packaging plasmids, investigating different...untreated and sucrose treated viral preparations.    Despite observing a positive effect on our overall yield...due to impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...ScFvs, based on community demand. Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...supporting hybridoma sharing within the community for decades. Educational Resources The Basics of Western Blotting...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ... to just that particular assay.      Fig. 1: A native protein (left) is denatured, allowing the...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...conditions. In those cases, pay special attention to designing your controls in validation, as you may need to...help you avoid a great deal of frustration when designing and troubleshooting immunoimaging assays. It can...

  7. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...year and happy reading! Top 10 blog posts of 2015 1. CRISPR 101: Homology Directed Repair Addgenie Chari...inversions, deletions, and translocations. It also provides researchers with many different ways to control...the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation process...experiment? In this post, Addgenie Chari Cortez guides us through some common controls and why you might...

  8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...do researchers track fluorescently labeled cells? 1. Epifluorescence microscopy This technique uses a ...of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals...their specificity or from the thymus to the lymph nodes. These methods gave researchers much more information...expressing cells. For example, by harvesting lymph nodes at different times after antigen challenge, scientists... cellular dynamics in lymphoid organs. In lymph nodes, for example, two-photon microscopy allowed scientists...extravasate from the bloodstream to invade lymph nodes and scan antigen presenting cells, but two-photon...interactions in diverse tissues, including lymph nodes, brain, liver, gut and skin. Two-photon intravital...

  11. Promoters

    Type
    Guide
    ...Strong mammalian promoter from human elongation factor 1 alpha CAG Constitutive Strong hybrid mammalian promoter...gene. The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from...Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA polymerase...

  12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... 160(1), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here...stepwise protocol on designing and using RNA scaffolds: Delebecque, C. J. et al. Designing and using RNA scaffolds...regulators allows a greater degree of control of desired expression levels dependent upon the type of transcriptional...transcriptional regulator or the number of designed scaffold binding sites. With hundreds of characterized...tunability of transcription is possible. From a design perspective, this is appealing since the RNA scaffold...pairing, allowing for rational, straightforward design. Since the technique relies on base pairing, it...and multidirectional multiplex control via scRNA design is readily apparent in metabolic engineering. The...

  13. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP ...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast...intracellular Ca2+ (bottom panels) equipped with destabilized GFP calcineurin reporter (blue) and stained ...

  14. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...and cost for AAV genome sequencing.  Figure 1: The viral genome sequencing workflow. Using this...our AAV preparations. We recently published a description of the VGS process and use cases in Human Gene...ligation and tagmentation preparation approaches described previously require a preliminary double stranding... the expression cassette that we want, it also includes a small amount of spurious DNA from the packaging...plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined serotype...signature sequences in the data. Unlike previously described thermodynamics-based methods of serotype confirmation...

  15. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...heterodimerizes with CIBN upon blue light exposure (see Fig 1*). When the group targeted the enhancer region of ...almost complete desensitization when exposed to continuous bright light. Desensitization is mediated by...discovered Marine, Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that...regulation of many genes. A number of groups have designed means of studying the effects of endogenous looping... they call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors...

  16. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently... What are epitope tags? Epitope tags are short peptides introduced at the N or C terminus of a protein...purification or detection of the tagged protein. These peptides consist of as little as 8 or as many as several...need monoclonal GFP antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential...Protein folding issues, steric hindrances, and destabilization can be brought about by the tag so it’s important...

  17. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    .... You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular...resources to provide them to their students. My desire to create a set of low-cost, easy-to-use educational...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable to many schools...students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in outputs that ...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...post about multiplexing with Cpf1.   Figure 1: Multiplexing allows researchers to express multiple...be used in a wide range of bacteria. References 1. Maddalo, Danilo, et al. “In vivo engineering of oncogenic...ligated into Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...promoters. To use this plasmid, you simply order oligonucleotides for your chosen gRNA target sequences and ...ordered assembly of multiple components into a destination vector. The first step in CRISPR/Cas9 Golden ...Golden Gate multiplexing is to clone the oligonucleotides specifying each gRNA target sequence into distinct...

  19. An Addgene Summer Internship

    Type
    Blog Post
    ...intern to complete and present on.    Figure 1: Addgene's summer interns presenting their capstone...instrument. They are the oldest sibling in a Guatemalan family of Mayan descent.   ...teaching me the basics of physics and sustainable design while allowing me to do my internship in the daytime...

  20. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...class.   Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a ...should also be easily understood and consistent in design and layout. 2. Be concise in your writing  When...
Showing: 521 - 540 of 704 results