We narrowed to 709 results for: esc

1. How-to: 5 Steps to a Great Panel Discussion

   Type

   Blog Post

   ...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...

2. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...reaction to this model on his blog. Or, for a description of how effective this model was in explaining...

3. Of Myc and Men

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   Blog Post

   ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

4. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design ...

5. Academic vs. Industry Postdocs

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   Blog Post

   ...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ... assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from entire...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

9. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...through several different applications such as fluorescent protein fusion studies and the generation of ...

10. Genome-wide Screening Using CRISPR

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    Blog Post

    ... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular...

11. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...matrix BLOSUM62, but you may want to check the description of the other matrices to see if another would...

12. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

13. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education...

14. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...diving cells will have higher HR frequencies than quiescent cells. It is important to know that HR is often...

15. DNA Purification Without a Kit

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    Blog Post

    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...

16. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...served. I hope that with some of the information described above you feel empowered to start your own journal...

17. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...

18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...need to use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the...

19. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ..., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly...

20. An Introduction to Adenovirus

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    Blog Post

    ...Early 3) genes deleted. These genes’ functions are rescued by helper plasmids used during production and/or...