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Showing: 521 - 540 of 625 results

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Plasmids 101: Choosing an Antibiotic Resistance Gene

Type

Blog Post

...express a gene contained in a plasmid. During the propagation and/or transformation process, some bacteria ...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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Blog Post

...VchINT was also shown to be active in various gram-negative bacterial hosts of interest, including within ...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...cysteines, including the native C547, allowing for ligation to pyridyl disulfide-activated ligands. Due to...
CRISPR Antimicrobials

Type

Blog Post

...resistant bacteria, allowing them to multiply and propagate their resistance genes. Our society is hoping ...
The Importance of a Fun Workplace: Company Culture at Addgene

Type

Blog Post

...up or cleanup, ask for volunteers to help out. Delegate- it will save you time and sanity! 10. Communicate...
Plasmids 101: Protein tags

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Blog Post

...Overexpression can also lead to insolubility, and aggregated protein is not useful protein. MBP tags can help...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...M153T, V163A Improves folding at 37C, reduces aggregation at high concentrations T203Y Red-shifts the...
Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...You can hover over the yellow or red circles to gather details on the issues associated with each sample...
Transferable Skills Guide: External Collaborations

Type

Blog Post

...connection by commenting and sharing. LinkedIn or ResearchGate allow you to send personal updates as messages...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells Int, 2016. 2016: p. 1329459. PubMed...
Transferable Skills Guide: Career Planning Resources

Type

Blog Post

... someone a lot older than you are; try using ResearchGate to see where other graduate students and post-docs...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

...focus on creating new antibiotic therapies, and investigate more effective drug targets (ones that are less...
Using Video to Share Your Science: We Share Science

Type

Blog Post

... with written publications Online tools like ResearchGate, GoogleScholar, Academia, ORCID, and MyScienceWork...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...
Supporting Reproducibility with a Connected ELN

Type

Blog Post

...available for public access, review, and query. Gates Open Research and Wellcome Open Research both accept...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

... Trombetta J, Sur M, Zhang F (2014) In vivo interrogation of gene function in the mammalian brain using...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...NGRRN Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus...
Hot Plasmids - August 2020

Type

Blog Post

... (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene! New...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

Type

Blog Post

...base pair edits of interest compared to cells segregated solely by a reporter of transfection, which only...
New CRISPR Tools: Cas7-11 and PASTE

Type

Blog Post

...lead to off targets and result in significant, negative effects in RNA targeting experiments. If Cas7-...

Showing: 521 - 540 of 625 results

Results per page:

Plasmids 101: Choosing an Antibiotic Resistance Gene

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

CRISPR 101: Ribonucleoprotein (RNP) Delivery

CRISPR Antimicrobials

The Importance of a Fun Workplace: Company Culture at Addgene

Plasmids 101: Protein tags

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Analyzing CRISPR Editing Results with ICE from Synthego

Transferable Skills Guide: External Collaborations

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Transferable Skills Guide: Career Planning Resources

Antibiotic Resistance: An Old Solution but a New Problem

Using Video to Share Your Science: We Share Science

New Tools Enable CRISPRa for Neuroscience Applications

Supporting Reproducibility with a Connected ELN

Overcoming the AAV Size Limitation for CRISPR Delivery

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Hot Plasmids - August 2020

Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

New CRISPR Tools: Cas7-11 and PASTE

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