We narrowed to 861 results for: lic
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TypeBlog Post...source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...technology that may be used for a variety of research applications, including making transgenic cell lines and ...
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A History of Genome Engineering in Popular Culture
TypeBlog Post...genetic code of living organisms and its societal implications even before the discovery of genes themselves... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...us to always consider the ethics and broader implications of our research. Want the Genome Editing Trading... -
Important Considerations When Using AAVs
TypeBlog Post...vivo animal model should consider the possible elicited immune response from viral infection. Animal safety...rate limiting step and can immediately undergo replication and transcription. However, with scAAV the effective...only can we compare these methods in specific applications, we may also be able to use these comparisons... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...Image from Doman et al., 2023, under a CC BY 4.0 license. epegRNAs: Structured RNAs with improved ... -
Antibody Validation for Flow Cytometry
TypeBlog Post...that the antigen being detected in the intended application — here, flow cytometry — is the desired target...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi.org/10.7554...of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. eLife... -
New Antibodies in the Lab Animation!
TypeBlog Post...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications. Thanks for watching, and we hope you found... -
New Videos: Addgene Lab Tips
TypeBlog Post...trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is ...the various reagents to a single tube and simply flick the tube several times. This will help ensure ensure... -
The Codon Guide to Synthetic Biology
TypeBlog Post...from Bruce Wetzel and Harry Schaefer, NCI, NIH | License I’m updating it regularly as people send me suggestions...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points... -
Antibody Data Repositories and Search Engines
TypeBlog Post... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any... -
Call for Guest Bloggers
TypeBlog Post...minipreps? If so, we’d love to have you write for us! Click Here to Contribute to the Addgene Blog Possible ...molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development... -
Summer Fun at Addgene!
TypeBlog Post... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of... -
Editor's Choice, July 2016
TypeBlog Post...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...in their own research. All posts from July 2016 Click on the links below to read all of our excellent ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post...foundational research, training, startups, and real applications of SynBio. Chapter 3: Synthetic biology as the...synthetic genome project and constructing ever more complicated biological devices. Chapter 6: - Making research... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant wells...using an antibody validated for your protein and application of interest or validating an antibody yourself...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...on the advisory board for Art in Giving, a 501c3 public charity raising funds for childhood cancer through... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... lend them many other functions for use in RNA splicing, imaging, and more. Abil says she is currently...says the new tool might even find therapeutic application in addition to their use in basic biology, for... -
Developing Transferable Skills During Science Training
TypeBlog Post... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal & Emily Walsh The Hard Truth About ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his... -
Easing the Protein Purification Process with pCri
TypeBlog Post...introduction to pCri, please see the original publication cited below. pCri plasmids are available either...comprehensive review of affinity tags and microbial applications." Biotechnology journal 7.5 (2012): 620-634.... -
Science as Art
TypeBlog Post... new method to explore the inner structures of delicate plants and other organisms without destroying ...competition. This competition has gained so much public interest for its stunning images that is has been...