...18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great ...

...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...

...the Wyss Institute - New CRISPR tools and the importance of moving on from "failed projects." 22:08 - ...

...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...photoswitchable” proteins offer exquisite spatial and temporal control of protein activity. Phytochrome, cryptochrome...expression. These plasmids rely on the engineered two-component regulatory system YF1/FixJ. YF1 is a synthetic...desynchronization of neural activity, with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185...K. 2011. Microbial opsins: a family of single-component tools for optical control of neural activity. ...

...synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use viral ...

...screens have been used to interrogate T cells, an important immune cell type, in more complex and physiological...

...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...

...popular blog post this month shares incredibly important information for anyone considering a postdoc. ...

...in your daily habits can feel ineffective or unimportant. With so many big contributors to the sustainability...

...36:53 - 38:00 : As an entrepreneur, is it more important to develop something that could succeed in the...

...out our blog post on CRISPR software to see the importance of programming for scientists!). Careers that...

...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...

...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...

...advancing career and professional development as core components of predoctoral and postdoctoral training. A grant...

... there is plenty more to explore. “It will be important to identify the key proteins and metabolites that...

... you properly visualize your results.The most important of those is to always look closely at the trace...

...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...no antibodies are available, change the least important factor (for instance, looking for a different ...

...visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting the Microbial...

...your top tips for science communication? It is important to identify your audience and consider their background...

...Software Engineer “Searching for a new job can be an imposing endeavor.  Break the process down into steps and...