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We narrowed to 985 results for: nin

Showing: 521 - 540 of 985 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...essential for proper muscle function. Without functioning dystrophin, an individual experiences progressive...skipping proceeds through the non-homologous end joining (NHEJ) pathway. It’s estimated that very little...Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome editing using...Used to Edit Genes Read How Non-Homologous End Joining is Used to Delete Genes Additional Resources on...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Stauffer, W. R., Fish, K. N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell...cells in C57BL/6J mice, but not in BALB/cJ mice, meaning that systemic capsids that rely on LY6A interactions...2017). AAV-PHP.eB has frequently been used for screening candidate regulatory elements such as enhancers...capsid of choice for future promoter/enhancer screening. However, AAV-PHP.AX packages viral genomes at...Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...cell (Dudek et al., 2018; Korneyenkov & Zamyatnin, 2021), meaning what works great in one system or cell...could help, or harm, your experimental goals when designing and testing your AAV.  Figure 2: Comparison...10.1016/j.omtn.2017.06.011 Korneyenkov, M. A., & Zamyatnin, A. A. (2021). Next Step in Gene Delivery: Modern...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... could try. We hope this post helped you start planning for your next multiplex IF experiment! If you ...Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., Bechtold, H., Dumitras, ...doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An ... Introduction to Performing Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp...

  5. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...they are the result of multiple plasmid copies combining in a process known as plasmid multimerization.... multimers grow more slowly compared to those containing the monomeric version of the plasmid (Summers...Multimers Run an undigested plasmid on agarose gel Running undigested DNA on a gel as a control when performing... 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one of the detection...

  6. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ... the cell, mitochondria play a vital role in maintaining cellular function. Mitochondria can also lend...capacity of cancer cells. This is certainly just the beginning for MitoTRACER, as it is a diverse tool that can...converting white adipose tissue (WAT) cells to brown or browning adipose tissue (BAT) — a type of fat tissue that...researchers developed a version of the kit that uses melanin production, allowing students to explore cell metabolism...

  7. Antibodies 101: Validation

    Type
    Blog Post
    ... the exciting new experiments that you’ve been planning, it is a good idea to pause and make sure that...approach may not be best if you are ultimately planning to use your antibody to detect endogenous levels...be a factor in your analysis. Example: You are planning an RNA-immunoprecipitation experiment and want...tuned for ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids...

  8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...enabled scientists to see how chaotic and random the scanning was and how fast T cells could jump from one antigen...neutrophils expressing LysM-GFP (green) into a draining lymph node after infection with S. aureus (red...-presenting dendritic cell subsets in the skin-draining lymph node." Proceedings of the National Academy...PMC4743831. 17. Cavanagh, Lois L., and Wolfgang Weninger. "Dendritic cell behaviour in vivo: lessons learned...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...Program (NIH-NIDA IRP), with the stated goal of learning behavioral optogenetics in order to implement ...types of constructs available, instructions for obtaining a material transfer agreement, and list of vendors... AAV construct is limited to only a few kb so attaining neuronal specificity through promoter-directed...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  10. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... editing to pooled library screening. It also has information for planning your CRISPR experiment. For...CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview and ...

  11. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are... An Introduction to Gating in Flow Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling...

  12. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve ... results at the bench, with a special focus on cloning and plasmid-based technologies. Commentary about...

  13. Our New Antibody Mascot is....

    Type
    Blog Post
    ...journey to name our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent...open until Feb. 22, 2022, and we'll unveil the winning name on Feb. 24. Stay tuned!    Original Blugene...your help naming our latest mascot, an antibody joining us on our scientific adventures.  You can submit...

  14. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...precluding analyses of T cell differentiation beginning with naïve T cells. The Framework for In vivo... is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker...Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering...

  15. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...researchers will add a lower case letter to the beginning of their insert abbreviation to specify what species...by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...the Glutamine at position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A...

  16. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...there something that we can invest our time into learning and extract the most value? This is only one of...might proceed in the future. When we think of designing experiments using CRISPR, there are two major ... general opinion. I could spend a lot of time running through each tool in detail, but that is already...the end of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing...

  17. Science Communication for Everyone

    Type
    Blog Post
    ...written for general audiences. So far, we are beginning to make an impact on society and science literacy... their questions and comments.  Lastly, we are planning to launch an e-magazine this year which would ...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...researchers - some readily found at Addgene. Shining light on transcriptional activation using dCas9...that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). This ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...spatially controlled and reversibly activated by turning blue light on and off. As one might expect from...

  19. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...gestures are energetic.  This can take practice and training. This is important because it creates a culture...feel that giving feedback will be perceived as complaining. They may worry that it seems like "tattling"...to feedback with your actions     Use active listening skills such as the PAC Method. P = demonstrate...

  20. Science as Art

    Type
    Blog Post
    ...just its biological importance. More of Daniel's stunning work can be found on his website http://www.microworldsphotography.com...with the need to conserve these unique samples, meaning that we know very little about these specimens ...competition has gained so much public interest for its stunning images that is has been featured in Time magazine...
Showing: 521 - 540 of 985 results