...cell (Dudek et al., 2018; Korneyenkov & Zamyatnin, 2021), meaning what works great in one system or cell...could help, or harm, your experimental goals when designing and testing your AAV.  Figure 2: Comparison...10.1016/j.omtn.2017.06.011 Korneyenkov, M. A., & Zamyatnin, A. A. (2021). Next Step in Gene Delivery: Modern...

... could try. We hope this post helped you start planning for your next multiplex IF experiment! If you ...Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., Bechtold, H., Dumitras, ...doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An ... Introduction to Performing Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp...

...they are the result of multiple plasmid copies combining in a process known as plasmid multimerization.... multimers grow more slowly compared to those containing the monomeric version of the plasmid (Summers...Multimers Run an undigested plasmid on agarose gel Running undigested DNA on a gel as a control when performing... 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one of the detection...

... the exciting new experiments that you’ve been planning, it is a good idea to pause and make sure that...approach may not be best if you are ultimately planning to use your antibody to detect endogenous levels...be a factor in your analysis. Example: You are planning an RNA-immunoprecipitation experiment and want...tuned for ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids...

...enabled scientists to see how chaotic and random the scanning was and how fast T cells could jump from one antigen...neutrophils expressing LysM-GFP (green) into a draining lymph node after infection with S. aureus (red...-presenting dendritic cell subsets in the skin-draining lymph node." Proceedings of the National Academy...PMC4743831. 17. Cavanagh, Lois L., and Wolfgang Weninger. "Dendritic cell behaviour in vivo: lessons learned...

...Program (NIH-NIDA IRP), with the stated goal of learning behavioral optogenetics in order to implement ...types of constructs available, instructions for obtaining a material transfer agreement, and list of vendors... AAV construct is limited to only a few kb so attaining neuronal specificity through promoter-directed...University.  His interests include the role of learning and memory in opioid addiction and science communication...

... editing to pooled library screening. It also has information for planning your CRISPR experiment. For...CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview and ...

...posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are... An Introduction to Gating in Flow Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling...

...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve ... results at the bench, with a special focus on cloning and plasmid-based technologies. Commentary about...

...journey to name our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent...open until Feb. 22, 2022, and we'll unveil the winning name on Feb. 24. Stay tuned!    Original Blugene...your help naming our latest mascot, an antibody joining us on our scientific adventures.  You can submit...

...precluding analyses of T cell differentiation beginning with naïve T cells. The Framework for In vivo... is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker...Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering...

...researchers will add a lower case letter to the beginning of their insert abbreviation to specify what species...by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...the Glutamine at position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A...

...there something that we can invest our time into learning and extract the most value? This is only one of...might proceed in the future. When we think of designing experiments using CRISPR, there are two major ... general opinion. I could spend a lot of time running through each tool in detail, but that is already...the end of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing...

...written for general audiences. So far, we are beginning to make an impact on society and science literacy... their questions and comments.  Lastly, we are planning to launch an e-magazine this year which would ...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com...

...gestures are energetic.  This can take practice and training. This is important because it creates a culture...feel that giving feedback will be perceived as complaining. They may worry that it seems like "tattling"...to feedback with your actions     Use active listening skills such as the PAC Method. P = demonstrate...

...researchers - some readily found at Addgene. Shining light on transcriptional activation using dCas9...that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). This ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...spatially controlled and reversibly activated by turning blue light on and off. As one might expect from...

...Resource sharing shortens the time needed to go from planning an experiment to performing one.  At Addgene, ...deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016). Many of the gRNAs... plasmids or from submission of a spreadsheet containing the validated gRNA sequences alone. Sequence ...

...just its biological importance. More of Daniel's stunning work can be found on his website http://www.microworldsphotography.com...with the need to conserve these unique samples, meaning that we know very little about these specimens ...competition has gained so much public interest for its stunning images that is has been featured in Time magazine...

...  Melina talked about her grandfather’s humble beginnings as an operator of a printing press. Just as her...Fan have been supporting Addgene from the very beginning and have played an integral part in its success...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...