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... editing to pooled library screening. It also has information for planning your CRISPR experiment. For...CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview and ...

...posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are... An Introduction to Gating in Flow Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling...

...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve ... results at the bench, with a special focus on cloning and plasmid-based technologies. Commentary about...

...journey to name our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent...open until Feb. 22, 2022, and we'll unveil the winning name on Feb. 24. Stay tuned!    Original Blugene...your help naming our latest mascot, an antibody joining us on our scientific adventures.  You can submit...

...precluding analyses of T cell differentiation beginning with naïve T cells. The Framework for In vivo... is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker...Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering...

...researchers will add a lower case letter to the beginning of their insert abbreviation to specify what species...by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...the Glutamine at position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A...

...there something that we can invest our time into learning and extract the most value? This is only one of...might proceed in the future. When we think of designing experiments using CRISPR, there are two major ... general opinion. I could spend a lot of time running through each tool in detail, but that is already...the end of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing...

...written for general audiences. So far, we are beginning to make an impact on society and science literacy... their questions and comments.  Lastly, we are planning to launch an e-magazine this year which would ...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com...

...researchers - some readily found at Addgene. Shining light on transcriptional activation using dCas9...that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). This ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...spatially controlled and reversibly activated by turning blue light on and off. As one might expect from...

...gestures are energetic.  This can take practice and training. This is important because it creates a culture...feel that giving feedback will be perceived as complaining. They may worry that it seems like "tattling"...to feedback with your actions     Use active listening skills such as the PAC Method. P = demonstrate...

...just its biological importance. More of Daniel's stunning work can be found on his website http://www.microworldsphotography.com...with the need to conserve these unique samples, meaning that we know very little about these specimens ...competition has gained so much public interest for its stunning images that is has been featured in Time magazine...

...  Melina talked about her grandfather’s humble beginnings as an operator of a printing press. Just as her...Fan have been supporting Addgene from the very beginning and have played an integral part in its success...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...

...have used fluorescent proteins to observe what’s happening inside cells for at least a couple of decades....tissues is set by the properties of hemoglobin and melanin pigments, which absorb the majority of light below..., thus, allowing higher resolution in samples containing substantial autofluorescence background.” Just...

... the beginning of the development of ARGs and have already developed a high-throughput screening method...Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts....

...Find Protocols Lab Tips to Keep Your Experiments Running Smoothly and "Gnome-Proof" This is why the “Bricking...science: The potential is endless One night, while opening a LEGO set with my husband, I thought about how... laboratory. It is priceless to see our “toys” gaining life and working as scientists in a custom made...

...pCMV-ABE7.10 has already earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR ...Repair mechanisms: CRISPR 101: Non-homologous End Joining and PITCHing MMEJ as an Alternative Route for Gene...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

... your job title doesn’t change. Master’s level training can prepare you to present your work at conferences...Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic v. Non-Academic...Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the Science...

...and it wasn’t long until the field was off and running with camelid-derived single-domain antibodies (...thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing...amenable to recombinant expression (and because maintaining a llama facility is out of reach and unappealing...

... us 8 short talks on every conceivable way of examining, modifying, and improving the CRISPR-Cas9 system...characterization and implementation of this system. Monday evening's talks switched gears (or should I say circuits...genetic circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic ...