AAVs CREATed for Gene Delivery to the CNS and PNS
Type
Blog Post
...-use AAV from Addgene here or check out these protocols if you want to package your own virus.
...
We narrowed to 658 results for: otos
-
-
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...acknowledging student apprehensions about “failure” (protocols not working, hypotheses being rejected, etc.),... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post... the bench, this results in a shorter staining protocol because the primary antibody can be “labeled” ... -
MXS Chaining
TypeBlog Post... Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1. Anderson JC, Dueber JE, Leguia ... -
CRISPR Antimicrobials
TypeBlog Post...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...later years, improvements to yeast transformation protocols also helped establish the yeast two-hybrid (Y2H... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...transformation, preparation, and analysis from our protocols and molecular biology reference pages! Resources... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...including a lot of future Addgenies as shown in the photo below. Kids and adults alike enjoyed some friendly... -
Plasmids 101: Protein tags
TypeBlog Post... function. More detailed information and some protocols can be found in the references provided. Additional... -
Transferable Skills Guide: Creativity
TypeBlog Post...these techniques to optimize our lab space, our protocols and even our Lab Inventory Management system. ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...Find ready-to-use AAV from Addgene or check out protocols for packaging your own virus ... -
Hot Plasmids - August 2020
TypeBlog Post...in E.coli as simple as following a DNA miniprep protocol! First, the authors created the pMAK backbone... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...distance runner, gardener, cook, and outdoor photographer. Additional resources on the Addgene blog ... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post... streamline your plasmid extractions. Use this protocol shared by Dr. Rob Hurt for high-throughput minipreps... -
Hot Plasmids Spring 2024
TypeBlog Post...nervous system research; however, microglia are notoriously resistant to viral transduction. Through in vivo... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...excluding the PAM sequence. PAM Sequence - The Protospacer Adjacent Motif (PAM) sequence for the nuclease... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...Social Networking for Scientists Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...fluorescent fusion proteins in cell biology."Current Protocols in Cell Biology (2005): 21-4. Pubmed PMID: 18228466... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...need share data with them including reports and protocols. To facilitate this, some ELNs allow you to invite...