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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 963 results for: tes

Showing: 521 - 540 of 963 results

  1. Plan Your Experiment

    Type
    Guide
    ...additional sites throughout the genome. These sites are called off-targets. Be aware of off-target sites when...efficiency. So while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific...that there could be downstream translation start sites that could allow for expression of a truncated protein...PAM sequences or Cas9 enzymes with flexible PAM sites . Base Editing : Target the site of your desired...complement to your target sequence with no homologous sites elsewhere in the genome. In reality, a given gRNA...genetic cargo (in this case, CRISPR components) integrates into the genome. Lentiviral vectors are a good...

  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may use it and modify it for non-commercial...

  3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...variety of research applications and particularly notes their ability to be precisely spatio-temporally ...down the HMO of interest, the lactose released activates expression of GFP from the lac operon. GFP expression...Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent Protein for Your Experiments Resources...

  4. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of the buffer...-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA fragments move ...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

  5. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...stood out from their initial testing. It outperformed other tRNA candidates (either from human or plant...muscular dystrophies, heart disease, and type 2 diabetes, across four different types of human cells, including...

  7. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further differentiated into dentritic...decisions about in vivo application and clinical testing. This would pave the way for improved patient outcomes...Burns-Naas, L. A. (2015). Chapter 27—Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis...

  8. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... that is probably the easiest path. Some institutes have structured visiting positions within defined ... any protocols the day before, etc.), take good notes, and ask questions if you don’t understand something...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    ...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...elevated at stable levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can...conditions better than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative...

  10. Components of CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR/Cas system, Cas9 is guided to its target sites with the aid of two RNAs: the crRNA which defines... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist of repeated...

  11. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... meeting on the calendar even if it is just 15 minutes a week. Clearly identify constraints and boundaries...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone...Update frequently with progress reports, meeting minutes and plan changes. Written documentation helps make...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...plasmid, and can be assayed conveniently. A direct test for your gene of interest may be another method ...always mission critical to make sure the plasmid replicates in the cell, as many of these experiments are... 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The concentration used for selection is...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...incompetent by using four plasmids instead of three and eliminates the requirement for Tat. Always includes deletion... transfer plasmids may have limited restriction sites or may only be compatible with certain cloning methods...target species. Although chromatin availability facilitates integration, it does not explain the lentiviral...more accurately predict and understand integration sites have led to the development of multiple genome-wide...al. (2020) for examples). Pseudotyping Tropism dictates which types of host cells the lentiviral vector...glycoprotein — transduce airway epithelium and cardiomyocytes in utero Rabies glycoprotein — transduce neural...for genome-wide mapping of lentiviral integration sites. Molecular Therapy — Methods & Clinical Development...

  14. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...calculated (details in [2]) and can be used to quantify rates of mutation in a bulk or single cell-derived genomic...instructions on how to design such assays, in addition to testing their performance (2). Remember that although digital...transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...

  15. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...below). Plating the transformation on ampicillin plates containing X-gal allows you to identify cells that...that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase. Complementing...this deleted portion, for example on a plasmid, creates a functional 𝛽-galactosidase. Blue-white screens...

  16. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al., 2018).  To create ...lab compared editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment...again that sgRNA structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They...

  17. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... start, the team inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry...SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this OptoNB-SOS fusion protein...membrane-localized mCherry. With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (...

  19. Hot Plasmids - May 2022

    Type
    Blog Post
    ... both on and off target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison...Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given that the...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...from CasPEDIA.   How can I use it? CasPEDIA operates like many search engines, featuring both a general...
Showing: 521 - 540 of 963 results