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We narrowed to 962 results for: tes

Showing: 521 - 540 of 962 results

  1. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further differentiated into dentritic...decisions about in vivo application and clinical testing. This would pave the way for improved patient outcomes...Burns-Naas, L. A. (2015). Chapter 27—Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis...

  2. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... that is probably the easiest path. Some institutes have structured visiting positions within defined ... any protocols the day before, etc.), take good notes, and ask questions if you don’t understand something...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may use it and modify it for non-commercial...

  5. Components of CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR/Cas system, Cas9 is guided to its target sites with the aid of two RNAs: the crRNA which defines... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist of repeated...

  6. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... meeting on the calendar even if it is just 15 minutes a week. Clearly identify constraints and boundaries...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone...Update frequently with progress reports, meeting minutes and plan changes. Written documentation helps make...

  7. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...plasmid, and can be assayed conveniently. A direct test for your gene of interest may be another method ...always mission critical to make sure the plasmid replicates in the cell, as many of these experiments are... 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The concentration used for selection is...

  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...to the right indicates a higher signal, or upregulation, and a peak to the left indicates a lower signal...continue gating populations of interest with normal gates, just based on a histogram plot instead of a dot...

  9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... packaging cell line. This system provides the greatest flexibility to pseudotype γ-retrovirus using different...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...U5 and the rest of the provirus. 3’ LTR Terminates transcription started by 5' LTR by the addition...

  10. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...companies or check the literature. Pro tip! Early gates, like your live/dead markers or commonly used markers...specific cell populations, often used in the later gates, are more likely to require FMOs. Isotypes Isotype...rather than for setting up your initial voltages and gates. Ashlyn has a research background in CD8+ T cells...

  11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...below). Plating the transformation on ampicillin plates containing X-gal allows you to identify cells that...that when a portion of the gene is deleted, it creates a non-functional 𝛽-galactosidase. Complementing...this deleted portion, for example on a plasmid, creates a functional 𝛽-galactosidase. Blue-white screens...

  12. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...repeat region on its 3’ end. This repeat region associates with the trans-activating crRNA (tracrRNA) which...at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the 2’ position...coupling several of them together, for example phosphorothioates with 2’-O-methyl modifications, proved more...

  13. Hot Plasmids - May 2022

    Type
    Blog Post
    ... both on and off target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison...Importantly, this is true in both mice and nonhuman primates (for AAV.CAP-B10, for example).  Given that the...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive...lineage, and so much more! Libraries allow you to test thousands of elements simultaneously in your system...dose. Vaccine development strategies. Image courtesy of Gorry et. al, 2007.   Clinical applications...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... trans activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...from CasPEDIA.   How can I use it? CasPEDIA operates like many search engines, featuring both a general...

  16. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...calculated (details in [2]) and can be used to quantify rates of mutation in a bulk or single cell-derived genomic...instructions on how to design such assays, in addition to testing their performance (2). Remember that although digital...transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...can rapidly and efficiently evaluate Cas9 cutting sites and activity (Knudsen et al., 2018).  To create ...lab compared editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment...again that sgRNA structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They...

  18. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... start, the team inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry...SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this OptoNB-SOS fusion protein...membrane-localized mCherry. With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (...

  20. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...together. I also prefer the number system to using dates as labels, since I often work on multiple projects...sure that the original code remains in tact! Take notes Comment in your scripts: Comments on what a given...who are still learning the basics. However, these notes take up a lot of space and don’t tell you why you...
Showing: 521 - 540 of 962 results