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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,046 results for: ins;

Showing: 271 - 280 of 1046 results

  1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...include proteins like nonstructural proteins 1 to 16, spike proteins, envelope small proteins, and membrane...membrane proteins. Although all viral components are available in bacterial expression plasmids, glycoproteins...glycoproteins and other proteins that require post-translational modification may not be easily expressed ...level expression and purification of SARS-CoV-2 proteins for downstream experiments and offer flexibility... doing things. These new processes have been instrumental in achieving our mission to help science move...Virus Entry Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana Press...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    ... 100 kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also...expressed proteins run in separate lanes. B: individually expressed and coexpressed proteins run as ladders...the Genetic Pertubation Platform at the Broad Institute have expanded the genetic screening toolkit by...express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli, either individually (~20,000 lanes...also available separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE...Western blots via engineered immunoglobulin binding domains.  Santilli, R.T., et al. Sci Rep (2021). https... large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and Cas12a, creates challenges for...

  3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...mice, exosomes expressed specific integrins (cell-adhesion proteins) targeting different organs. This ...action outside of tumor cells, and recent studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely...physiology is deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts...whole, microenvironments promote tumor durability instead of repressing them, making it even harder to cure...possibly inducing the secretion of growth promoting proteins, such as human growth factor, vascular endothelial...microenvironment itself. They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell...

  4. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...recognizing the target protein remains bound to the column while the rest of the proteins are eluted. Then, the...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...the serum are separated, and the serum - which contains the antibodies - is further processed to isolate...Protein G purification is one common method. These proteins bind the IgG heavy chain. Running the serum over...specific to the immunogen or not, while any other proteins pass through. However, protein A and protein G...Antibodies that don’t cross-react with the serum proteins pass through the column, resulting in a polyclonal...or select a monoclonal or recombinant antibody instead. Otherwise, you might experience variability in...

  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...amount of information regarding which regulatory proteins, signaling molecules, mutations, and environmental...represented in a very small number of registers. Instead, one may use qPCR to determine the fraction of ...combination of red, blue, and green fluorescent proteins in each state. The three methods of state interrogation...tell you the distribution of states in a sample; instead, it will suggest the predominant state of registers...plasmid (high copy, ColE1 origin of replication), contains large serine recombinase genes downstream of inducible...copy, bacterial artificial chromosome backbone) contains the register. Depending on the desired application...endogenous gene expression (2). Our paper (1) explains how to arrange attB-attP pairs on a register to...

  6. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...Massachusetts Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the... the Karolinska Institutet, among others). Mentors and mentees held scheduled monthly progress video conferences... confined by birth country or financial means. Instead, talent should be realized through equal opportunities...internationalization faces several obstacles; for instance there are few resources to help get individuals...supported, and can more fully identify with and be inspired by the mentor’s successful example. For evidence...2014-15, University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional...universities and high level public and private institutions also showed their support and hope for the IMP...

  7. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...sharing their worm strains through the Caenorhabditis Genetics Center (CGC) which maintains and distributes...each half to a gp41-1-N-intein. Split inteins are protein domains that naturally “associate, self-excise...distributes the strains all over the world. When I was at the 22nd International C. elegans meeting, I was again... the Arizona State University (ASU) Biodesign institute developed a 3’UTR database specifically for C.... discovered in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al., 2015)...modified TIR-1 protein can degrade degron-tagged proteins in an auxin dependent manner. This system allows...scientists to both spatially and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL...

  8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription...RNAs (ncRNAs) are transcribed but do not encode proteins. They play important roles in many cellular and...permanently disable, protein-coding genes by generating insertions or deletions in the DNA sequence. This same approach...break works well because very small deletions and insertions in the DNA sequence can completely disrupt expression... an open reading frame, so small deletions or insertions may not result in a functional loss (Ho et al...the ncRNA (Hazan and Bester 2021). For example, inserting a polyadenylation signal downstream of the transcription...exon or a large genomic fragment. (D) Knock-in: insertion of a synthetic polyadenylation (spA) signal. Figure...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    ...AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific AAV delivery Recombinant anti-FLAG...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare (Preprint...alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance By Hridaya Patel (Addgene co-op...systemic gene delivery in non-human primate (NHP) brains.  Systemic gene delivery capsids previously identified...

  10. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...families of cell surface and secreted proteins (glypicans, integrins, and more coming soon) as well as epitope...engineer the most miniscule parts of life, like proteins, to study or alter their function for a variety...Degenerate primers are a group of primers that contains a variety of degenerate codons (different codon...amino acid) for various amino acids. Essentially, instead of forcing sequence diversity using error-prone...guided directed evolution might be more your style. Instead of leaving sequence generation to chance, you start...regions you want to evolve. With this added layer of insight, you can shrink your mutant library and increase...binding specificity, you can perform binding assays against the target antigen to identify mutants with enhanced...
Showing: 271 - 280 of 1046 results