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Showing: 811 - 840 of 1027 results
  1. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...of Toronto. He is an author of 20+ scientific publications and patents.   ...
  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note...
  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...times."     - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading...
  4. The MTA at Addgene

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    Blog Post
    ...things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar agreements...portal automates a lot of the process, from notifications and emails to generating the UBMTA link for ...
  5. How to Lead a Great Meeting

    Type
    Blog Post
    ... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ...
  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages...
  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...
  8. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...
  9. Antibodies 101: Isotypes

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    Blog Post
    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of these five isotypes, such as the number and location of disulfide bonds, the number of constant domains...
  10. Plasmids 101: Blue-white Screening

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    Blog Post
    ...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR...
  11. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...studying autophagy. This method relies on the identification of autophagic structures based on morphology...
  12. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post
    ... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...
  13. Transferable Skills Guide: Problem Solving

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    Blog Post
    ...might even be expected to write up a report to communicate your findings to your colleagues. So perhaps ...about the issue, assessed potential protocol modifications, and ultimately decided on the changes to implement...
  14. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....
  15. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...
  16. Developing Lab Management Software for Biology

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    Blog Post
    ...for a scaleable lab. When you need to track the location, quality, growth, and legal status of thousands...stab culture Ship the stab culture to the proper location To keep ourselves from mixing up samples and ...
  17. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    .... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...
  18. Working with Nuclear Receptors

    Type
    Blog Post
    ...murine members. NRs permit the integration and communication of such signals between central and peripheral...macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa ...
  19. Lab Automation at Addgene

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    Blog Post
    ...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...
  20. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...accomplishments and demonstrate your science communication skills. Know your audience. Connect your prior...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...
Showing: 811 - 840 of 1027 results