We narrowed to 1,027 results for: Cat
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TypeBlog Post...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...of Toronto. He is an author of 20+ scientific publications and patents. ...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note... -
Celebrate National Book Month with Addgene
TypeBlog Post...sometimes we don't." - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...times." - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...plasmids at Addgene Opperman J et al., Nature Communications, 2019. PubMed PMID: 31346176. Using LADL to...light-activated-dynamic-looping (LADL) system. The group fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they... -
The MTA at Addgene
TypeBlog Post...things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar agreements...portal automates a lot of the process, from notifications and emails to generating the UBMTA link for ... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...research papers or dissertations. Phrases are categorized either based on the section of a manuscript they...introduction, methods, results; or phrases are categorized by the goal of the phrase, such as being cautious... -
How to Lead a Great Meeting
TypeBlog Post... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...beforehand on which chromosome your docking site is located). This amounts to a small difference of two or ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color... -
Antibodies 101: Isotypes
TypeBlog Post... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of these five isotypes, such as the number and location of disulfide bonds, the number of constant domains... -
Plasmids 101: Blue-white Screening
TypeBlog Post...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...great way to get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about ...tapping into it and helping make it work by being an educated mentee. Other opportunities: Try virtual mentoring... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...studying autophagy. This method relies on the identification of autophagic structures based on morphology... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...mini-grant, which will fund a project to support a dedicated website to host all the materials in one place...for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...tegmental area (VTA) of the brain. Since the VTA is located in the midbrain, fiber-optic cannulas were still...right secondary motor cortex (M2). Since M2 is located on the surface of the brain, to deliver light to... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...might even be expected to write up a report to communicate your findings to your colleagues. So perhaps ...about the issue, assessed potential protocol modifications, and ultimately decided on the changes to implement... -
What's New in CRISPR - November 2021
TypeBlog Post...AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease.... -
All in a Twist: dsRNA
TypeBlog Post...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ... -
Developing Lab Management Software for Biology
TypeBlog Post...for a scaleable lab. When you need to track the location, quality, growth, and legal status of thousands...stab culture Ship the stab culture to the proper location To keep ourselves from mixing up samples and ... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post.... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or... -
Working with Nuclear Receptors
TypeBlog Post...murine members. NRs permit the integration and communication of such signals between central and peripheral...macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa ... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...Rescue occurs through guanidinium cation (Gdm+) binding in the location formerly occupied by the Arg sidechain... -
Lab Automation at Addgene
TypeBlog Post...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities... -
How to Prepare for an Industry Interview
TypeBlog Post...accomplishments and demonstrate your science communication skills. Know your audience. Connect your prior...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...between undergraduate school and studying for the MCAT, she decided that she didn’t want to take it. “I...to your values While going through the co-op application process, my advisor encouraged me to keep my ...