We narrowed to 650 results for: CHI;
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TypeBlog Post...In this system, light activates the neurons (via oChIEF) and a chemogenetic agonist inhibits the neuron...
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Antibodies 101: Fab Fragments
TypeBlog Post... A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...several methodologies currently available for approaching it. These include the traditional restriction... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post... inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...were assessed and could improve over time. They achieved this by administering pre- and post-workshop surveys... -
To Each HIS Own
TypeBlog Post... blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to Antibodies References... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...inducible expression (more on this below) will both achieve temporary repression of protein expression. Addgene... -
All in a Twist: dsRNA
TypeBlog Post...foreign dsRNA activates the innate immune response machinery of the host. This pathway has been clinically... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...arbitrary DNA sequences and have been shown to achieve ~100 fold repression in E. coli. In addition, TALEs... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...enough. We also had to enforce and maintain it. Teaching people to use words in a new way is not necessarily... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...continual advancements in gRNA design necessary to achieve maximal on-target and minimal off-target activities... -
The Future of Research Symposium Boston 2015
TypeBlog Post...example, in 2010, less than 15% of postdocs had achieved tenure-track positions within 5-7 years of earning... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post... hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed by Rui... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post... sgRNA to temporarily block the transcription machinery of a target gene or DNA that codes for ncRNA. ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...strategy. A donor plasmid contains 1 kb homology arms matching the gene of interest, a linker, and the GFP tag... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...Mike Morrison, a psychology doctoral student at Michigan State University. The idea is that the main research... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...genotypic levels. The editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%...