We narrowed to 772 results for: Dos

...eukaryotes don’t naturally have this response, they do contain the specific ubiquitin ligase pathway needed...

...read of the paper is much more detailed. I prefer to do it on another day - preferably the following morning...

...We began our research with a simple question: how do Cas9 and sgRNA interact with and dissociate from ...

...transparency in an otherwise messy process.  And you do have options for the terms you want to share your...

...third parties or your home institute. Discuss rights Do you expect to generate data that will contribute ...

...- but for many other proteins, quality antibodies do not exist, or they don’t exist for a specific application...

...indirectly probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing a similar ...

...main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are receiving...

...think of entrepreneurship in the 21st century, who do you think of?  Your mind is likely drawn to the Mark...

...multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires multiple...

...vector by excising the insert from the backbone. To do this, you'll use enzymes with restriction sites that...

...would would make a good fit for me?" Whatever would I do next and what options lie ahead of me? Like a considerable...

...Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves...

...sites — similar to restriction enzyme cloning. To do this, your DNA fragment must first be amplified with...

...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable with...use in yeast, as parts of biosenors, and much more. Do you have a favorite use for PA-FPs? Let us know in...

...the histogram plots look a bit different than they do for yes-no readouts.      Figure 4: A histogram...

...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities...

... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene...

...chromosomal loci in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to...

...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides...