Addgene

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Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...
The Importance of a Fun Workplace: Company Culture at Addgene

Type

Blog Post

... ordinary environment Team building events like Escape the Room or Habitat for Humanity take you and your...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...
An Inside Look into Addgene's Dev Team

Type

Blog Post

... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...
Bioinformatics at Addgene

Type

Blog Post

...of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...
New CRISPR Tools: Cas7-11 and PASTE

Type

Blog Post

...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts Resources...
CRISPR Activation: A Practical Guide

Type

Blog Post

... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...
The Challenges of Cell Culture

Type

Blog Post

...free of contamination and match their original description. The oldest, largest, and most diverse of these...
Plasmids 101: Gateway Cloning

Type

Blog Post

...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...
Viral Vectors 101: Preparing Pooled Libraries

Type

Blog Post

...excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...
Career Insights: Technical Support Specialist

Type

Blog Post

..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Type

Blog Post

...vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...don't see protein overexpression? The strains described above should generate sufficient expression levels...
Data Freedom: The Expansion of Data Sharing in Research Publications

Type

Blog Post

...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...
Plasmids 101: Biotinylation

Type

Blog Post

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

Showing: 541 - 560 of 705 results

Results per page:

Negative Can Be Positive: Open AAV Data with Addgene

The Importance of a Fun Workplace: Company Culture at Addgene

Overcoming the AAV Size Limitation for CRISPR Delivery

An Inside Look into Addgene's Dev Team

Bioinformatics at Addgene

New CRISPR Tools: Cas7-11 and PASTE

Antibodies 101: Immunoprecipitation

CRISPR Activation: A Practical Guide

The Challenges of Cell Culture

Plasmids 101: Gateway Cloning

Viral Vectors 101: Preparing Pooled Libraries

Plasmids 101: Stringent Regulation of Replication

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Career Insights: Technical Support Specialist

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Changing Labor Laws Bring Increased Postdoc Wages

Important Considerations in Optogenetics Behavioral Experiments

Plasmids 101: E. coli Strains for Protein Expression

Data Freedom: The Expansion of Data Sharing in Research Publications

Plasmids 101: Biotinylation

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