We narrowed to 703 results for: ESC;

...Early 3) genes deleted. These genes’ functions are rescued by helper plasmids used during production and/or...

...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

...Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education...

...need to use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the...

... signal amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed...

...diving cells will have higher HR frequencies than quiescent cells. It is important to know that HR is often...

...Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, ...

...expected results for the three primers previously described. When using insert-specific primers (1), positive...

...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...

... ordinary environment Team building events like Escape the Room or Habitat for Humanity take you and your...

...of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...

...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative...

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  Resources...

...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...

... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

...excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction...

...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...