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We narrowed to 628 results for: ESP

Showing: 541 - 560 of 628 results

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Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Type

Blog Post

... Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar works led to a Nobel ...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput experiments...
Plasmids 101: Optimizing Plasmid Yields

Type

Blog Post

... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles...
Experimenting with New Careers while in Grad School

Type

Blog Post

...excellent way to meet people with similar interests! Especially as someone interested in doing science outreach...
PITChing MMEJ as an Alternative Route for Gene Editing

Type

Blog Post

...proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh is a robust...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g...
Plasmids 101: Multicistronic Vectors

Type

Blog Post

...Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

... people to use the Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make...
MXS Chaining

Type

Blog Post

...expression and detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining...
Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

...life cycle. One should ponder this figure often, especially the three steps from the 12 o’clock to the 6 ...
Uncovering Ancestral Bias in CRISPR Research

Type

Blog Post

...variability. Moving forward It took a five-team response to address this issue, but it still points to ...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...you to lose soluble proteins from the sample, especially if left on for too long or used at too high a...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...colleagues who read it with me, the consistent response from scientist and non-scientist alike was that...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...
DNA Purification Without a Kit

Type

Blog Post

....1371/journal.pone.0007750 Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...vector with restriction enzymes and adding the corresponding homologous sequences to the insert by PCR. ...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

...mono-methylation, tri-methylation, and aceylation, respectively (these are chemical modifications, or functional...

Showing: 541 - 560 of 628 results

Results per page:

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Fluorescent Tagging of Endogenous Genes with SapTrap

Plasmids 101: Optimizing Plasmid Yields

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Experimenting with New Careers while in Grad School

PITChing MMEJ as an Alternative Route for Gene Editing

Light Sheet Fluorescence Microscopy

Plasmids 101: Multicistronic Vectors

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

MXS Chaining

Viral Vectors 101: The Retroviral Lifecycle

Uncovering Ancestral Bias in CRISPR Research

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

CRISPR 101: RNA Editing with Cas13

Capturing a Moment with Marc Zimmer’s “The State of Science”

SciComm: Taking Science to Elementary and Middle Schools

DNA Purification Without a Kit

Simplify Cloning with in vivo Assembly

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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