Plasmids 101: Degron Tags
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Blog Post
...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...
We narrowed to 639 results for: ESP
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Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...fluorescent molecules conjugated together) can be especially sensitive to batch effect. For example, when ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar works led to a Nobel ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput experiments... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post... then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...commonly FPs with red and green wavelengths, respectively, are selected (6). These previous blog posts... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed mK-GO somewhat... -
Transferable Skills Guide: Managing a Team
TypeBlog Post.... They will make mistakes and you will take responsibility (within reason!). Try to buffer them from ... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles... -
Experimenting with New Careers while in Grad School
TypeBlog Post...excellent way to meet people with similar interests! Especially as someone interested in doing science outreach... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh is a robust... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post... people to use the Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...accurate measure of DNA concentration. This is especially problematic in low-concentration samples (samples... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...colleagues who read it with me, the consistent response from scientist and non-scientist alike was that... -
DNA Purification Without a Kit
TypeBlog Post....1371/journal.pone.0007750 Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post...life cycle. One should ponder this figure often, especially the three steps from the 12 o’clock to the 6 ...