We narrowed to 639 results for: ESP

... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking...

...Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles...

...excellent way to meet people with similar interests! Especially as someone interested in doing science outreach...

...proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh is a robust...

...limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g...

...Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always...

... people to use the Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make...

...vector with restriction enzymes and adding the corresponding homologous sequences to the insert by PCR. ...

...expression and detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining...

...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...

...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit...

...colleagues who read it with me, the consistent response from scientist and non-scientist alike was that...

...life cycle. One should ponder this figure often, especially the three steps from the 12 o’clock to the 6 ...

...variability.   Moving forward It took a five-team response to address this issue, but it still points to ...

...you to lose soluble proteins from the sample, especially if left on for too long or used at too high a...

...accurate measure of DNA concentration. This is especially problematic in low-concentration samples (samples...

....1371/journal.pone.0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification...

...mono-methylation, tri-methylation, and aceylation, respectively (these are chemical modifications, or functional...

...amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise that researchers...

...you used for cloning. This type of control is especially important for insert-specific primers. If your...