We narrowed to 637 results for: ESP

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   ...fluorescent molecules conjugated together) can be especially sensitive to batch effect. For example, when ...

2. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput experiments...

3. Cloning Mammalian Cells with the Agarose Method

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   ... then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work...

4. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...commonly FPs with red and green wavelengths, respectively, are selected (6). These previous blog posts...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed mK-GO somewhat...

6. Transferable Skills Guide: Managing a Team

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   .... They will make mistakes and you will take responsibility (within reason!).  Try to buffer them from ...

7. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar works led to a Nobel ...

8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ... Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org...

9. Plasmids 101: Optimizing Plasmid Yields

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   ... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles...

11. Experimenting with New Careers while in Grad School

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    ...excellent way to meet people with similar interests! Especially as someone interested in doing science outreach...

12. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh is a robust...

13. Light Sheet Fluorescence Microscopy

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    ...limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g...

14. Plasmids 101: Multicistronic Vectors

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    ...Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ... people to use the Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make...

16. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ...accurate measure of DNA concentration. This is especially problematic in low-concentration samples (samples...

17. Viral Vectors 101: The Retroviral Lifecycle

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    ...life cycle. One should ponder this figure often, especially the three steps from the 12 o’clock to the 6 ...

18. Uncovering Ancestral Bias in CRISPR Research

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    ...variability.   Moving forward It took a five-team response to address this issue, but it still points to ...

19. CRISPR 101: RNA Editing with Cas13

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    ...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit...

20. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...colleagues who read it with me, the consistent response from scientist and non-scientist alike was that...