We narrowed to 671 results for: FIE

...836-42. PubMed PMID: 28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J....

...proteasome degradation. Autophagy is then induced and quantified by measuring the amount of radioactivity in the...

... your lab bench, we need to (this is a little simplified): Find the appropriate glycerol stock within...

... constantly improving. Cas9 variants have been modified for genome editing, activating gene expression...

...both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this precise...

...results when the plasmid is linearized. Treat the purified AAV samples with DNase I to eliminate any contaminating...

...of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold was found to ...

...Resources page.  CasPhi is 70 kDa Cas protein identified exclusively in huge bacteriophage genomes. It...

...activity using light or drugs have been developed, the field lacked an “off-switch” for Cas proteins. Discovery...

...popularity – and notoriety – as a tool of choice in the field: dCas9 fused to the enzymatic domain of TET1 or ...

...multiple steps in which a precursor molecule is made, modified (sometimes many times), and isolated afterwards...

...schematic. Created with BioRender.com. The pegRNA specifies both the genomic location to be edited and the...

.... (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level. Adenovirus is similar...

...our instrument and begin the analysis. We use a modified version of the Python program described by Joung...

...with the goal is to generate mice that have two modified genes in their genome, and assuming Mendalian ...

...through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help...

...al. "Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors." Nature communications...

... And finally, provide feedback. If you are unsatisfied with the nature or speed of a response, let your...

...nucleases and NgAgo is an important next step for the field. Based on what we know now, Cpf1 may be the best...

...Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid Collections! Join the Addgene...