We narrowed to 672 results for: FIE

1. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference from Cas9...

2. Antibodies 101: Affinity Reagents

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   ...situations where a suitable antibody has been identified but a smaller or less immunogenic version is ...

3. Transferable Skills Guide: Problem Solving

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   ...resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset...

4. Antibodies 101: Polyclonal Antibodies

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   ...recognizing your protein of interest: affinity-purified antibodies are isolated from the serum by their...

5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...to form a protein identical in sequence to the unmodified enzyme. Each half of the base editor is fused...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ... constantly improving. Cas9 variants have been modified for genome editing, activating gene expression...

7. Plasmids 101: Golden Gate Cloning

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   ...both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this precise...

8. Viral Vectors 101: Virus Safety

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   .... (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level. Adenovirus is similar...

9. Lab Automation at Addgene

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   ...through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help...

10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...al. "Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors." Nature communications...

11. Design Tips for Prime Editing

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    ...schematic. Created with BioRender.com. The pegRNA specifies both the genomic location to be edited and the...

12. Tips for Technical Support Calls

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    ... And finally, provide feedback. If you are unsatisfied with the nature or speed of a response, let your...

13. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...nucleases and NgAgo is an important next step for the field. Based on what we know now, Cpf1 may be the best...

14. Plasmids 101: Modular Cloning

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    Blog Post

    ...multiple steps in which a precursor molecule is made, modified (sometimes many times), and isolated afterwards...

15. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...with the goal is to generate mice that have two modified genes in their genome, and assuming Mendalian ...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...popularity – and notoriety – as a tool of choice in the field: dCas9 fused to the enzymatic domain of TET1 or ...

17. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...our instrument and begin the analysis. We use a modified version of the Python program described by Joung...

18. Hot Plasmids and Viral Preps - January 2021

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    ...of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold was found to ...

19. Hot Plasmids - October 2020

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    ...Resources page.  CasPhi is 70 kDa Cas protein identified exclusively in huge bacteriophage genomes. It...

20. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...activity using light or drugs have been developed, the field lacked an “off-switch” for Cas proteins. Discovery...