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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 856 results for: HA

Showing: 541 - 560 of 856 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Cecchetelli The auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to...light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...in the messaging stream. Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect...easy favor for people in your network. If someone has connected with me, I consider them fair game for ...

  4. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ...darken during the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape to label...

  6. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Further, Sanger sequencing has a lower detection limit of 50-20% (although this has been improved in some ...Joung 2016 with permission. Even though progress has been made with off-target prediction algorithms, ...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...syndrome. In response, the World Health Organization has declared a global health emergency and called for...molecular and electronic companion technologies that has transformed the assay’s capabilities. Specifically...

  10. A Look at Addgene's QC Process

    Type
    Blog Post
    ...including the propagation of plasmids in bacteria — has many points at which small errors can be introduced...Catching and addressing QC issues Addgene’s QC team has developed a process for identifying, categorizing...

  11. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...categorization is complex Plasmid classification has shifted over the years, and new classifications are...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many...

  12. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally...important — but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available...

  17. Hot Plasmids - October 2020

    Type
    Blog Post
    ...generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene...
Showing: 541 - 560 of 856 results