...generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene...

...AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure your gene goes where...research. To improve cell-type specificity of AAV, work has focused on modifying the outside protein shell, or...

...to host proteins. After the antibody of interest has been cloned into an expression plasmid, the plasmid...through open plasmid sharing, the CRISPR community has created hundreds of useful variants of Cas9. By providing...

...anyone who has ever worked to sequence DNA on NGS platforms will tell you, while the process has become much...

...including the propagation of plasmids in bacteria — has many points at which small errors can be introduced...Catching and addressing QC issues Addgene’s QC team has developed a process for identifying, categorizing...

...categorization is complex Plasmid classification has shifted over the years, and new classifications are...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many...

...available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally...important — but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available...

...particles with polybrene, a cationic polymer. Polybrene has long been used to increase retroviral transduction...of mouse zygotes and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice...

...syndrome. In response, the World Health Organization has declared a global health emergency and called for...molecular and electronic companion technologies that has transformed the assay’s capabilities. Specifically...

...training. Addgene executive director Joanne Kamens has written a 5-part blog on the topic:  http://blog....people mostly online and on monthly phone calls. This has its challenges as meeting in person is often much...

...Professor Goedhart is very active on Twitter and has helped us update many of our educational resources...

...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...

...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to ...

...we highly recommend checking out the video). Tom has made many more Science rap videos to teach students...

...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along...

...injection before performing the experiment. Every entry has an accompanying image so that you can see for yourself...

...used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters...

... generation systems because the packaging vector has been divided into two separate plasmids (resulting... transcription of the full-length virus after it has incorporated into a host cell. The potential for ...practices with regard to lentiviral research. The NIH has additional information on lentiviral safety considerations...

...isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant DNA technology...isolates, the K-12 strain and the B strain. K-12 has led to the common lab strains MG1655 and its derivatives...polymerase. Thus to replicate a piece of DNA in vitro one has to know some of its sequence to design a effective...Epitope Tags Tag Amino Acid Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST...

... crucial environmental research programs, Canada has been here before.  These attacks on science ultimately...politicians saying that climate change is a hoax. This has culminated in the current President holding and campaigning...the sacrifice in April because devaluing science has alarming implications for our public policies—many...