We narrowed to 877 results for: HA

...which is now easily available through Addgene and has already been requested dozens of times in 2 weeks...Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain, making...

...editor in 2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities...contexts. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...

...easily assemble multiple DNA fragments. This system has been used to construct plasmids for use in model ...Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized for its biotechnological...

...on holistic, community-centric science, our team has continuously engaged with a variety of stakeholders...Undergrad category at the iGEM Competition 2019. He has an active interest in scientific writing and science...

...For example, the Levin lab from Tufts University has used Arch (green-light activated proton pump) and...Allen Discovery Center. The future of this field has a high potential for the advancement of research ...

...Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor...allosteric inhibitors at Novartis. The ClonTracer library has been distributed all over the world and was recently...

...functional elements in the human genome. The pair has focused considerable attention on transcription factors...the tagged transcription factor. The FLAG antibody has been well characterized and the system allows you...

...HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...ends interfere with that step. Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...can be manipulated at one time. Weissman’s group has made standard protocols available and they are working...

...base editing to pooled library screening. It also has information for planning your CRISPR experiment. ...

...prime editing By Emily P. Bentley The David Liu Lab has made two recent deposits focused on optimizing prime...collaboration with the Vallabh/Minikel Lab, the Weissman Lab has developed a new epigenetic editor, Coupled Histone...subtypes, and a familiar challenge for anyone who has done histology on brain tissue is identifying the...

...well if you’ve been following the Addgene blog, it has been repurposed for genome editing in eukaryotes,...editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (Zhang et al., 2016...than that, be nice to your microinjectionist, who has the strategically important job of delivering the...

...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...including creating Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method...left homology arm guanine (i.e. GG); this design has been shown to increase T7 promoter activity. To avoid...

...served by a one-sample test as the standard value has no variation that needs to be accounted for. ANOVA...multiple range test, and several others. Each test has different likelihoods of returning false positives...to a single group. Dunnett’s is more powerful and has a lower error rate than the multiple comparison tests...

... a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while...for all amplicons; if your amplicon is AT-rich or has a secondary structure, you may see a double peak ...being a common choice - the frequently used GAPDH has noted issues as a reference gene (Higgins, 2000; ...assumptions that match your data set, that is.) Yuan, 2006 has a good overview of different statistical analysis...

...-homologue recombination was released. This blog has not been updated in response to this paper. Any hint... hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published...

...The characterization of RNA-protein interactions has lagged behind, likely due to limitations of current...interactions. Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding...

...can be pretty complicated given that a single cell has tens of thousands of proteins functioning in a variety... would not be able to do. We hope that this blog has answered all of your questions about polyhistidine...

...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

...Cecchetelli The auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to...light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA...