We narrowed to 851 results for: Met
-
TypeBlog Post...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...people) -> How can you make a decision about something like this without sufficient data? Starting to...
-
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post..., peer-review is a touchstone of the scientific method, ensuring that published work meets the accepted...manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator system...Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127. PubMed... -
Intro to Our Summer SciComm Series!
TypeBlog Post...communication itself, not the communication method, that determines if something is scientific or science communication... -
RUBY-Red Siliques
TypeBlog Post... instead, which can’t be done if your selection method kills the transgene free individual. For example...studying large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post... Why recombinant antibodies? Animal-based methods for antibody production have several drawbacks....neuroscience research today, but there is a critical unmet need for an open-access, molecularly defined, and... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...single particle creates many copies of itself, sometimes up to millions of new copies, which are released... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...Isotype matters when constraining using the indirect method since a secondary antibody reactive to a certain... not be used for multiplexed indirect staining methods since the secondary antibody used will react to... -
Plasmids 101: TOPO Cloning
TypeBlog Post...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...sensitive to sunlight and prone to developing metastatic melanomas and other cancers. Joshua Wang (bottom...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...unwavering devotion to seeing your ideas become something tangible. When you think of entrepreneurship ...heavy price tag, especially if you're working on something other than software. If you are a graduate student... -
Another Pathway into Cells: iTOP
TypeBlog Post...description of the lab’s iTOP protein-only transduction method and its potential applications to CRISPR/Cas9 genome...osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies showed that... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...in college that I really fell in love with it. Something about being able to actually hold all those seemingly...educational uses. We believe that biology education is something that should be available to everyone, and hope... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...simplifies the process of building advanced multi-parametric search queries. Looking for a bright, monomeric...emission peak around 600nm? Simply input these parameters and you’ll find matching FP suggestions. If ... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...near-infrared photosensitizer. Nature Methods,13(3), 263-268. doi:10.1038/nmeth.3735. PubMed PMID: 26808669. PubMed... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...Ribosome profiling Ribosome profiling, a biochemical method developed in 2009, takes a “snapshot” of all of...UTR were translationally silent. Both of these methods increase our understanding of translation dynamics... -
Addgene Depositors Get More Citations
TypeBlog Post... & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary journals...represent different research areas, target readers, methodologies, or qualities of research our adjustment controls... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...sequencing have prevented laboratories from using these methods for routine processes like plasmid validation. ... problematic for conventional NGS library prep methods because the yield or quality of individual libraries... -
Science Career Options
TypeBlog Post...Sales Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting...Lab Manager Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post... 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP to track...pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab...