...find useful antibodies for your research. Please consider sharing your experiences with antibodies from .... The content of this article is solely the responsibility of the authors and does not necessarily represent...

...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...

...our early stage.  After each pitch we made, we considered the feedback and adjusted our business model ...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...

...iTOP is best suited to binary systems in which transient protein expression has a long-lasting effect, ...in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or the potential...

...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...network Attend informational interviews to gain insight into the daily routine of the potential job Join...

... check to make sure that it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase...opens in a new window) NEB's table of methylation sensitive restriction sites . Sometimes enzymes cut sequences...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....

...production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often...the outside surface of the exosome or are carried inside the vesicle, which may also contain other cellular...

...manipulate neural populations in order to get new insights into functional network interactions. Combining...bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim” in the...

... Everyone. Plan out new experiments carefully. Consider submitting a registered report, where peer review...is based on the methods, not the results. Also consider using this framework to design your future experiments...

... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary... (PostgreSQL), that aims to eventually be a comprehensive community resource for information about fluorescent...

...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ...

...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter... to make her work understandable to people both inside and outside the scientific community. We’re excited...

... However, the pH changes in our solutions are transient and return to normal once the tube is opened and...restored. We have no evidence to suggest that the transient change in pH affects AAV function. Furthermore...

...for specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action... variant 1513-WPRE (105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary...

...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success...

...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher...

...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...

...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...