We narrowed to 651 results for: PPL

1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...which can then be used for downstream cloning applications. While these two strains ultimately perform ...

2. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some...

3. Addgene's Tips for Plasmid Quality Control

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   ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ...

4. The Future of Research Symposium Boston 2015

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   ...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ...

5. Plasmids 101: Cre-lox

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   ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

6. Design Tips for Prime Editing

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   ...its design changes for every new prime editing application. And optimizing its sequence is important, as...

7. Making CRISPR Plasmids Using Fragmid

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   ...validated and were chosen because of their broad applicability in different model systems. What’s the error...

8. Antibodies 101: Stripping and Reprobing Western Blots

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   ...retaining residual antibodies. If you have already applied antibodies and need to dry the membrane for storage...

9. CRISPR in the Clinic

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   ...disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult stem cells...

10. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...

11. Viral Vectors 101: Chemogenetics

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    ...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

12. Viral Vectors 101: Virus Safety

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    ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required and...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...excitation or inhibition upon light (or drug) application.  Genetic manipulation can also reduce expression...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...time. Antibodies made from animals are limited in supply and producing antibodies from plasmids is much ...

15. Hot Plasmids and Viral Preps - March 2021

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    ...from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody pairs. Because the...

16. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed PMID: 12917490. PubMed...

18. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1...

19. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...of competitive TED talk – to win the audience’s applause with your performance. When your institute opens...

20. Fluorescent Proteins 101: Introduction to FRET

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    ..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...