We narrowed to 653 results for: PPL

1. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...which can then be used for downstream cloning applications. While these two strains ultimately perform ...

2. Addgene's Tips for Plasmid Quality Control

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   ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ...

3. The Future of Research Symposium Boston 2015

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   ...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ...

4. Plasmids 101: Cre-lox

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   ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

5. Hot Plasmids and Viral Preps - January 2021

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   ...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

6. Hot Plasmids - October 2020

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   ...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

7. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...B, Bondy-Denomy J (2020) Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies....

8. CRISPR in the Clinic

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   ...disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult stem cells...

9. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...

10. Viral Vectors 101: Chemogenetics

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    ...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

11. Viral Vectors 101: Virus Safety

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    ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required and...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...

13. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed PMID: 12917490. PubMed...

14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1...

15. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...excitation or inhibition upon light (or drug) application.  Genetic manipulation can also reduce expression...

16. Design Tips for Prime Editing

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    ...its design changes for every new prime editing application. And optimizing its sequence is important, as...

17. Making CRISPR Plasmids Using Fragmid

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    ...validated and were chosen because of their broad applicability in different model systems. What’s the error...

18. Antibodies 101: Stripping and Reprobing Western Blots

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    ...retaining residual antibodies. If you have already applied antibodies and need to dry the membrane for storage...

19. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...trials support the utility of AAV as a broadly applicable gene delivery tool, aspects of AAV biology should...

20. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...of competitive TED talk – to win the audience’s applause with your performance. When your institute opens...