We narrowed to 609 results for: SHE

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...

3. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ...vector. For example, although researchers know that Usher Syndrome (a disease that results in combined deafness...

4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

5. An Introduction to Adenovirus

   Type

   Blog Post

   ...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

6. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

7. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   ...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

8. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

9. CRISPR Antimicrobials

   Type

   Blog Post

   ...antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize...

10. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    ...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...

11. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    ...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single...

12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    ...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...

13. Lentivirus Production

    Type

    Protocol

    ...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

14. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...might help to get you started. Once you have established a well working protocol in your lab, it can become...

15. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....

16. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    .... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...

19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    ...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

20. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type

    Blog Post

    ... Alyssa Shepard...