We narrowed to 577 results for: Vit

1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

2. Treating Muscular Dystrophy with CRISPR Gene Editing

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   ...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...

3. How to Negotiate a Successful Lab Start Up Budget

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   ...opinions on creating and negotiating budgets and invite you to leave a comment below Happy Sciencing, ...

4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   ...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis...

5. Antibodies 101: Multiplex Immunofluorescence

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   ...handling steps, or there’s some unexpected cross-reactivity. Keep in mind that you may need to optimize the...

6. CRISPR 101: Off-Target Effects

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   ...by Cas does not always correlate with catalytic activity at those sites, especially when DNA mismatches...

7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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   ...regulation, as long as they can control recombinase activity. For example, post-transcriptional recombinase...

8. PiggyBac-ing Through the Genome Editing Field

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   ...repeats (ITRs) found on the piggyBac™ donor for its activity. In summary, the piggyBac™ transposon is a powerful...

9. Plasmids 101: Restriction Cloning

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   ...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

10. Finding Your Perfect Job After University

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    ...have meetings between teams. This communication is vital because otherwise I’d lose touch with my colleagues...

11. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln...

12. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low levels...

13. A Guide to Getting Started in Undergrad Research

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    ...When you are hunting for labs to join, one of the vital things to look for is a supportive environment—it...

14. Important Considerations When Using AAVs

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    ...virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15...

15. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...particularly interested in science communication and in vitro diagnostics.     References 1. Costantino N, Court...

16. With an Eye Towards the Future, We Look Back at the March for Science

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    ... clinic regulations, evidence-based research is vital in combating misinformation and propaganda that ...

17. Antibodies 101: Introduction to Antibodies

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    ...sequences can be optimized for specificity and sensitivity, for example by changing certain amino acids ...

18. Typing CRISPR Systems

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    ...called CasPEDIA, with information about enzyme activity, experimental conditions, and more. Type II Type...

19. Antibodies 101: Designing Your First Flow Panel

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    ...lab. Let's say I am culturing HEK 293T cells in vitro. I transduced the cultured cells with a virus that...

20. CRISPR 101: Multiplex Expression of gRNAs

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    ...knockout or edit. This can help reduce off-target activity. Deleting a large region of the genome by removing...