Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 949 results for: ache

Showing: 541 - 560 of 949 results

  1. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...routine. Set a goal to read a certain number of papers each week to keep up with the research in your area. ...literature in your field of interest. Set aside some time each day or week to at least scan the titles of what’...alerts from journals you read often. The contents of each issue will be delivered to your inbox as soon as...

  3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... Ophthalmology Basel recently took a different approach to enhance specificity in retinal cells. To do...type specific expression in the retina.  Four approaches for designing synthetic promoters  To build a...permission. In total, this library contained 226 AAVs, each with a different synthetic promoter driving expression...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    ...running professional development (PD) workshops for teachers; organizing Congressional briefings in Washington...eugenics and research misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the ...non-technical writing skills by writing for science-outreach organizations or student publications, submitting...

  5. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...can express siRNAs to direct the cellular RNAi machinery to silence target genes. This empty MuLV vector...plasmids (based on the Murine Stem Cell Virus, MSCV) each with a unique 6 nucleotide barcode. This collection... Susanna Bachle...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...thoroughly decontaminated after use, typically with bleach (plastic) or ethanol (other surfaces). For AAVs...and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine are useful. Some organizations or...policies – basically, you’ll need to get trained in each new place (and pay attention to the trainings!),...

  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...to probe the blot again, almost like new. This approach preserves your sample and saves you from having...your sample. Pro tip! Because some protein is lost each time you strip a blot, you should not compare the...cycles! Just remember that a bit of sample is lost in each round of stripping, so it pays to plan ahead.   ...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...it is being inserted: one thousand base pairs on each side, in fact. Later, when we transfect the cells...also find the certificate of analysis provided with each cell line. The editing strategy and design described...

  9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Relations Board to allow graduate students who work as teaching or research assistants to join unions. The topic...provide a better deal for students in areas such as teaching salaries, healthcare access, and benefits; however...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...outlining the different steps and materials required for each [1, 2]. This protocol [3] provides, in exquisite... appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources...the laser must be controlled. As discussed above, each opsin variant has slightly different activation/...

  11. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...longer term and more substantial experience can be achieved by volunteering for a professional organization...organized a “Committee Leadership Institute” (CLI) where each Committee Chair and Vice Chairs were invited to ...was ultimately invited to join the Advocacy and Outreach Committee within a professional society in my ...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table ...future in. And we decided to make education and outreach a cornerstone of our business, because we are ...flowers, bugs, and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from ...

  13. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...it is also a good idea to run an uncut sample of each vector to help with troubleshooting if your digests...approximately 1:3. Since the number of base pairs for each varies, it is difficult to calculate this based ... alone. One method is to conduct 2 ligations for each plasmid you are trying to create, with varying ratios...

  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...confluent because cells like their space. If they reach confluence, they stop growing (contact inhibition.... Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent...pipettes for liquid waste) should be sterilized before each use. Also, all contaminated liquid and solid waste...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...pBAD vectors for example. Protein expression from each of these vectors is controlled by a different promoter...resulting in different levels of expression from each vector; lower expression may be required if your...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...

  16. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...it is critical to include an unstained sample for each tissue type you are running in your panel if you...

  17. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus...releasing the virus to the supernatant. The virus will reach peak production when roughly 60–70% of cells have...

  18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Addgene AAV Production protocol, I take a lazier approach for harvesting my AAV. I skip the PEG precipitation...immediately before iodixanol purification. This approach gives me a bit of flexibility since I can store... Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and purification! References...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... through the specimen, which often leads to photobleaching.  Furthermore, point-by-point scanning along...focus light. Therefore, there is much less photo-bleaching or photo-damage than in conventional laser scanning...215. Pubmed. W. Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten...
Showing: 541 - 560 of 949 results