We narrowed to 830 results for: eed

1. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   ...in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite...understand where sonic hedgehog got its name, we need to go back to 1980, when Christine Nusslein-Volhard...

2. CRISPR-mediated Plant Base Editors

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   ... gene Y, which has a defective allele Ym and you need to convert an adenine (A) to a guanine (G)? You ...with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112.11 (2015...

3. Hot Plasmids - August 2020

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   ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! ...

4. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ... noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside...Some of the variability was organic, as some people need less assistance than others, but we did have some...

5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination should...induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance...

6. Antibodies 101: Reading a Flow Plot

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   .... But in order to plan a flow experiment, you’ll need to first read papers with flow data... and to read...endless loop. Let’s break out of the loop with all you need to know on how to read a flow plot.  Dot plots ...

7. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   ... adjacent motif) near the target DNA, TnpBs also need a short sequence motif called a TAM (transposon-... used to generate gene-edited plants without the need for tissue culture (Maher et al., 2020; Qiao et ...

8. Hot Plasmids: Fall 2024

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   ...processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression proteins...co-imaging. And because bioluminescent markers do not need to be excited by an external light source, they ...

9. Twenty Years of Sharing: Addgene's Viral Vector Service

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   ...viral vector selection process to standardize our feedback and evaluation of viral vectors. This process ...as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest a viral vector for...

10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ... with WPRE reduced the number of copies of gvpA needed to increase expression. We were able to express...microscopy and TEM (Fig. 2c), and ultrasound imaging. If needed, resort the cells again for improved purity and...

11. Career Insights: Technical Support Specialist

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    ...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing...

12. 5 Reasons to Use Reddit for Science Communication

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    ...Submission Guidelines here, but essentially we just needed to send a Google calendar invite over to the r/...a paid account which gives me all the features I need to save, organize and easily find the resources ...

13. Tips for CRISPR Gene Editing in Mice

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    ...you may even wish to generate a point mutation and need to focus your attention to that particular region...easier and doesn’t require the extra precautions needed when dealing with RNA (e.g. face mask, and a dust...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...or C-terminal fusion based on your experimental needs. Equally important, before cloning you must consider...dark fusions is likely misfolded, blocked from proceeding through the secretory pathway, and retained in...

15. Important Considerations in Optogenetics Behavioral Experiments

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    ...neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison of... to use c-fos expression to get the evidence you need. For example, as shown in this study [3], hippocampal...

16. Plasmids 101: E. coli Strains for Protein Expression

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    ...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...

17. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...their biotinylation. The advantage of APEX is its speed, labeling neighboring proteins in minutes rather...developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous biotin ligases...

18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded and therefore...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

19. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ... A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence. The idea was that these “blocking mutations...locus you are editing, and if the blocking mutation needs to be silent. In our view, it’s always best to change...

20. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...