We narrowed to 821 results for: eed

...in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite...understand where sonic hedgehog got its name, we need to go back to 1980, when Christine Nusslein-Volhard...

... gene Y, which has a defective allele Ym and you need to convert an adenine (A) to a guanine (G)? You ...with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112.11 (2015...

...Submission Guidelines here, but essentially we just needed to send a Google calendar invite over to the r/...a paid account which gives me all the features I need to save, organize and easily find the resources ...

...you may even wish to generate a point mutation and need to focus your attention to that particular region...easier and doesn’t require the extra precautions needed when dealing with RNA (e.g. face mask, and a dust...

...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing...

...Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination should...induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance...

...2014). Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these...Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher...

... with WPRE reduced the number of copies of gvpA needed to increase expression. We were able to express...microscopy and TEM (Fig. 2c), and ultrasound imaging. If needed, resort the cells again for improved purity and...

.... But in order to plan a flow experiment, you’ll need to first read papers with flow data... and to read...endless loop. Let’s break out of the loop with all you need to know on how to read a flow plot.  Dot plots ...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! ...

... noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside...Some of the variability was organic, as some people need less assistance than others, but we did have some...

...or C-terminal fusion based on your experimental needs. Equally important, before cloning you must consider...dark fusions is likely misfolded, blocked from proceeding through the secretory pathway, and retained in...

...neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison of... to use c-fos expression to get the evidence you need. For example, as shown in this study [3], hippocampal...

...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...

...Lentiviral vectors can be produced easily, without the need for time-consuming purification processes, and are...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...

...In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded and therefore...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

... A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence. The idea was that these “blocking mutations...locus you are editing, and if the blocking mutation needs to be silent. In our view, it’s always best to change...

...their biotinylation. The advantage of APEX is its speed, labeling neighboring proteins in minutes rather...developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous biotin ligases...

...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

...between cells can rescue aerobic respiration. Proceedings of the National Academy of Sciences USA, 103(... system (Figure 6). This approach eliminates the need for expensive reagents and equipment like incubators...