We narrowed to 709 results for: esc

1. CRISPR 101: Making a Knock-In Cell Line

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   Blog Post

   ...diving cells will have higher HR frequencies than quiescent cells. It is important to know that HR is often...

2. Lentiviral Vector Uses and Overview

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   Blog Post

   ...Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, ...

3. Plasmids 101: Colony PCR

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   Blog Post

   ...expected results for the three primers previously described. When using insert-specific primers (1), positive...

4. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   Blog Post

   ...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...

5. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   ... ordinary environment Team building events like Escape the Room or Habitat for Humanity take you and your...

6. An Inside Look into Addgene's Dev Team

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   Blog Post

   ... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

7. Plasmids 101: Gateway Cloning

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   Blog Post

   ...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

8. Changing Labor Laws Bring Increased Postdoc Wages

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   Blog Post

   ...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   Blog Post

   ...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...

10. Career Insights: Technical Support Specialist

    Type

    Blog Post

    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...

11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ...vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ...

12. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...

13. Bioinformatics at Addgene

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    Blog Post

    ...of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...

14. QC Sequencing Technologies at Addgene

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    Blog Post

    ...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

15. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

16. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

17. Plasmids 101: Stringent Regulation of Replication

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    Blog Post

    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...

18. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post

    ...excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction...

19. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative...

20. Antibodies 101: Immunoprecipitation

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    Blog Post

    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  Resources...