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Showing: 541 - 560 of 876 results
  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...
  2. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic Molecular Biology. Elsevier, pp ...for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR...
  3. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A "complex" system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...
  4. An Introduction to Adenovirus

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    Blog Post
    .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...Isolation of a Cytopathogenic Agent from Human Adenoids Undergoing Spontaneous Degeneration in Tissue ...
  5. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post
    ...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 ...
  6. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post
    ...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...
  7. CRISPR Activation: A Practical Guide

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    Blog Post
    ...et al., 2018). cDNA libraries, which consist of plasmids that over-express coding sequences from a given...Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins...
  8. An Inside Look into Addgene's Dev Team

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    Blog Post
    ... development includes everything from tracking plasmids from the moment a deposit kit is sent to a depositor...improvements, you are benefited with reassurance your plasmids will not get lost in a freezer and your viral ...
  9. Tips for Using FRET in Your Experiments

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    Blog Post
    ...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent...downloads/modules/FRET_teaching_module.pdf Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: ...
  10. 10 Steps to a Perfect Science Talk

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    Blog Post
    ...professionally). Do remember the fine points of visual aids. Here’s a great slideshare with more details on ...question. I like to ask about how many people use plasmids in their research and then see how many of them...
  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...
  12. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ... recombinant adeno‐associated viruses." Nucleic acids research 32.1 (2004): e3-e3. PubMed PMID: 14704360...endogenous genes in diverse human cell lines." Nucleic acids research 36.19 (2008): e127-e127. PubMed PMID: 18784188...
  13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral...
Showing: 541 - 560 of 876 results