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We narrowed to 985 results for: nin

Showing: 541 - 560 of 985 results

  1. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...  Melina talked about her grandfather’s humble beginnings as an operator of a printing press. Just as her...Fan have been supporting Addgene from the very beginning and have played an integral part in its success...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...

  3. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...have used fluorescent proteins to observe what’s happening inside cells for at least a couple of decades....tissues is set by the properties of hemoglobin and melanin pigments, which absorb the majority of light below..., thus, allowing higher resolution in samples containing substantial autofluorescence background.” Just...

  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...pCMV-ABE7.10 has already earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR ...Repair mechanisms: CRISPR 101: Non-homologous End Joining and PITCHing MMEJ as an Alternative Route for Gene...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  6. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...Find Protocols Lab Tips to Keep Your Experiments Running Smoothly and "Gnome-Proof" This is why the “Bricking...science: The potential is endless One night, while opening a LEGO set with my husband, I thought about how... laboratory. It is priceless to see our “toys” gaining life and working as scientists in a custom made...

  7. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ... your job title doesn’t change. Master’s level training can prepare you to present your work at conferences...Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic v. Non-Academic...Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the Science...

  8. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...and it wasn’t long until the field was off and running with camelid-derived single-domain antibodies (...thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing...amenable to recombinant expression (and because maintaining a llama facility is out of reach and unappealing...

  9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ... us 8 short talks on every conceivable way of examining, modifying, and improving the CRISPR-Cas9 system...characterization and implementation of this system. Monday evening's talks switched gears (or should I say circuits...genetic circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic ...

  10. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...Managing a team is hard and most people have some learning they need to do before they can be good at it....diversity of responsibilities, which might include training or teaching others, research, and policy development...can move to a new workplace if opportunities for learning and personal growth do not exist or if they feel...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    .... Minigenomes are stripped down viral genomes containing only the sequences required for viral replication...CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly...barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make...

  14. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...and while we think of the AAV vector genomes as containing just the expression cassette that we want, it...plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined serotype...two types of adeno-associated virus particles containing complementary polynucleotide chains. J Virol ...

  15. Summer SciComm: Science Storytelling

    Type
    Blog Post
    .... You can introduce your results right at the beginning of your communication, use conflict to get your...really dig into the significance of the results, explaining what new questions are next or how this will ...impact future research or policy.  It is worth mentioning that this is far from the only way to communication...

  16. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...abstract protein during your sample prep process, imagining how it starts and if any steps could affect its...conditions. In those cases, pay special attention to designing your controls in validation, as you may need to...help you avoid a great deal of frustration when designing and troubleshooting immunoimaging assays. It can...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... (also referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems... biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted...library targeting S. pneumoniae D39V from Jan-Willem Veening is now available with one sgRNA per operon or gene...

  18. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...knock-in at a genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you...peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged amino acids...need monoclonal GFP antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential...

  20. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...after 6 years in the business of western blots and cloning, I decided to pack it in. I had just started month...Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me as a product and not me as...I had no previous business, marketing or sales training and knew I had to start somewhere. I knew if I...
Showing: 541 - 560 of 985 results