... about Addgene. “The mission to share critical reagents such as plasmids and viral stocks for researchers...otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier, PhD Frank Stegmeier...targets that have the biggest potential to transform patients' lives. Previously Stegmeier was the Head of Oncology...see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

...They provide accounts of their research struggles in papers. They recount accomplishments in talks and... pay attention. Highlight your lowest points and highest points and make clear what was difficult and ...you don’t like it yourself. Be proud of your achievements and accept and acknowledge your failures. Foster...

...academia, it is especially important for graduate students and postdocs to get a perspective on other types...use of resources and pay attention to budget constraints Academic science is much like industry but the...science’s sake More opportunity to train and teach students Collaborations may be less “complicated” - for...tell you to pick up the pace Constant worry about grants and renewals What is different about non-academia...apple can change entire culture) Specific requirements for hiring more common Likelier to hire on ...

...monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color...therefore can be used in immunoprecipitation experiments with other fluorescent proteins. Find mNeonGreen... coli with a gene library of approximately 484 variants, including some potential tetramer-breaking mutations...a protein which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and...

...publication that could advance treatments for a rare disease Adult patients or caregivers affected by the... others partner with formal learning to engage students at all levels.      Jargon is field-specific terminology...minorities in your local high school who are also students in AP biology classes Creating a podcast about...explicitly exclude people outside of the original recipients. Instead, it targets a specific audience - giving...

...able to order ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining...applications, enabling you to better plan your experiments. To promote reproducibility, Addgene will have...resource may also expand to include other affinity reagents, such as nanobodies and ScFvs, based on community...applications. In addition, basic and advanced affinity reagents topics are discussed on Addgene’s blog and Antibody...recombinant antibodies or other recombinant affinity reagents, share your plasmids via Addgene! Email deposit...

...grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved in ...weeks and with labs worldwide closing for unknown amounts of time, we were facing a financial crisis. As ... resource for scientists that helps them share reagents — leading to open, reproducible science,” said...More scientists are sharing plasmids and other reagents every day, and this crisis has highlighted the...

...professional societies hosting virtual networking events. Download Addgene’s Science Career guide to learn...reproducibility best practices Support your future experiments and other researchers by implementing reproducibility...out Reproducibility for Everyone. Plan out new experiments carefully. Consider submitting a registered report...consider using this framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search ... the AAV Data Hub for tools to use in future experiments. If you’ve used Addgene’s AAV tools, you can ...

...culture and in live zebrafish. For cell culture experiments, only dL5**-expressing cells treated with MG-...neighboring bystander wild-type cells.   Zebrafish experiments with MG-2I-dL5** were performed with embryos ...Zebrafish are an ideal model organism for these experiments because developing embryos are transparent, so...also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed to YFP and RFP...your experiences using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato...

...digested with ribonucleases, ribosomes protect the segments of mRNA to which they are bound. These regions...color switching, TRICK requires the following components: a GFP protein that binds to the coding region...the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry labeled mRNA and keeps...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these changes ...

...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...first design is a whole cell biosensor with two components - an enzyme that breaks down the HMO of interest...communication skills throughout her training and wants to make her work understandable to people both inside...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein...

... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute...particularly prominent problem in CRISPR genome editing experiments. We aim to alleviate this frustration by producing...primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's...our viral services. Please send any questions or comments to [email protected]. Finally we’d like to thank...

...while sipping an afternoon coffee in between experiments. The longer we are in this flexible work schedule...people use is the built-in whiteboard, in which participants can contribute and sketch on the board without...time breaks up the monologue a little and gives participants something else to focus on. Use collaborative... creativity are pivotal in writing publications/grants too. As described by several works (Wuchty et al...be done randomly or manually assigning which participants to group together. Breakout Rooms can be used...

... inactive dCas9 molecules. We followed these experiments with more detailed investigations into the release...delivery by nucleofection The in vivo editing experiments in this publication were performed using a technique...well as the sequences of our validated editing reagents can be found on our plasmid pages. We encourage...encourage any labs performing genome editing experiments to try nucleofection as (in our hands) this technique...transfection approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9...

... this system to develop a bacterial camera that prints a chemical image, as well as a genetic method for...experimented with the promoter strength of various components, removing inducible promoters that could crosstalk...increased dynamic range to around 100-fold. These improvements will allow researchers to better fine-tune the... input light intensity. In addition to these improvements, the constructs have been greatly streamlined...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over...

...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...documented history in science. Potential graduate students typically go through a rotation, which reveals...we do fits in with what they want to do. The experiments must fit with their interests.” George Church.... Alternatively, perhaps the lab publishes and presents regularly with labs around the world. You may ...the happy dance at least once in awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College...

...race to find vaccines, therapeutics, and other treatments to stem the spread of COVID-19. That’s why we...you used plasmids from this collection in your experiments and published your data, please fill out this...expression vectors containing various spike protein variants are also available. Plasmids are continually added...proteins, and membrane proteins. Although all viral components are available in bacterial expression plasmids...purification of SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage, codon...

...and editing speed and consists of the following components: A plasmid in the host bacterium expressing ...infectious. The editing speed dictates which phage variants can propagate faster than the rate of dilution...uninfected host cell culture). This dilution step represents the major difference between PACE and PANCE: ...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

...controlling cell signaling. Key components of OptoNBs OptoNBs have two key components: an AsLOV2 domain and a ...activity in cells.  Let’s shed some light on the key components of OptoNBs, how they are generated, and their...variable heavy chain antibody domain.  How do these components interact? When fused together to form an OptoNB...below table summarizes the results for these experiments.  AsLOV2 Inertion Site mCherry #1 mCherry ...

...involving protein stability or binding experiments. Potential experiments that one might perform using the ...transcripts for degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is ...the specific gene producing long unspecific RNA fragments that are incorporated into the dsRNA necessary...membranes, organelles, and other subcellular compartments is via proteomics--the study of the distribution...