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  1. CRISPR Cheat Sheet

    Type
    Blog Post
    ...resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult...
  2. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...some great costumes this Friday. So if you’re interested in finding out who wins this year’s Addgene costume...
  3. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies will grow...E. coli cells by heatshock with the plasmid of interest. See our transformation page for a detailed heatshock...
  4. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...#ChristmasScene #womenwhoscience #scigirls #wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication...
  5. New Viral Vectors - March 2024

    Type
    Blog Post
    ...JEDI-2P, deposited by the St-Pierre lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (...
  6. Editor's Choice, July 2016

    Type
    Blog Post
    ...have any questions or useful insights. If you're interested in blogging for us, fill out our Guest Blogger...
  7. Addgene's Website Update

    Type
    Blog Post
    ...search box to go straight to your materials of interest!   Figure 1: The new search bar.   Pro tip! You...
  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...telling a story makes scientific topics both interesting and approachable!   The grand finale of visual...
  9. General Transfection

    Type
    Protocol
    ...transfect mammalian cells with your plasmid of interest....Transfections allow for transient expression of a gene of interest in a target cell line and can be useful for short...
  10. Gibson Assembly Protocol

    Type
    Protocol
    ...extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost any two fragments regardless...and screen for the correct plasmid product by restriction digest . Sequence the important regions of your...
Showing: 541 - 560 of 755 results