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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,046 results for: ins;

Showing: 281 - 290 of 1046 results

  1. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim...optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These resources can now be...Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin...used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang...and find plasmids containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for ...Fluorescent Vegetables in Aptamer Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences...shares some strategies for generating GFP fusion proteins. From considering protein size and shape, linker...

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...plasmid's journey begins First, each package is opened and the contents are carefully inspected to verify that...different strains of E. coli that can be used for transformation. The preferred E. coli strains for storage... then the journey of transformation and storage begins. Some samples are submitted as bacterial colonies...the year (see graph below). Addgene scientists inspect each sample and make sure they have been barcoded...assigned to a location in a metal freezer rack that contains five levels that can hold five plates on each ... back into because every barcode on every tube contains information about that tube’s appropriate location...been received and stored, it is put into E. coli strains that have been treated to easily take in plasmids...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...the surface or the inside of your cells. While proteins themselves can emit intrinsic fluorescence when...amino acids found in all proteins, so you can't distinguish the different proteins from each other. To distinguish...Lastly, there are fixable dye stains that react with free amines on and inside the cells and are thus typically...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach...appropriate readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such... reporter's fluorescence. Another way to label proteins is through treating cells with fluorophore-conjugated...antibody-fluorophores, fluorescent reporters, and/or dye stains used in your flow cytometry experiment are known...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... of Antonello Bonci, Director of the National Institutes of Drug Abuse Intramural Research Program (NIH-NIDA...are numerous variants for the different types of opsins available and their differences and uses have been...discussed thoroughly elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University...details about types of constructs available, instructions for obtaining a material transfer agreement,...published at this point.  In brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2...and subsequent firing of an action potential. Halorhodopsins (HR) are green/yellow-light sensitive chloride...specific opsin expression. However, the types of opsins you are able to use are obviously limited by the...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... Chavez, researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously...and former researcher at the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex)...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is...similar tools were made using other DNA binding proteins such as zinc fingers (ZF) and TAL effectors (TALE...scale CRISPR-based screens can be used to gain insight into protein structure and function (Pan et al....Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti...Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM (2015) Highly efficient Cas9-mediated...

  6. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...and find an ideal antibody against Protein A and an ideal antibody against Protein B. The antibodies are...isotype? Antibodies are composed of light chains and heavy chains made up of variable regions and constant...interact. You read extensive research on the two proteins and come up with a great experimental plan that...detect Protein B and successfully visualize both proteins.     Figure 2: Two primary antibodies ...In most cases, the resulting chimeric antibody retains the binding specificity to the original target ...target and can be used with secondary antibodies against the new Fc region. In addition to increasing multiplexing...     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,...

  7. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...microbial strains. It proved to be impractical to catalog every variant of a feature. Instead, we identified... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...their goals as intuitive and painless as possible. Instead of crowding the interface with every possible option... antibiotic resistance markers and replication origins, but there is extensive heterogeneity in the feature...efforts, such an algorithm has limitations. For instance, it could miss a common feature if the sequence... inaccurately. The best example is fluorescent proteins, which often come in closely related versions ... genomes of newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to ...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ... generally preferred for large insertions such as fluorescent proteins or selection cassettes. This class...conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et...Stroik January 24, 2023. DNA damage drives genome instability and contributes to cancer, premature aging, and...sticky or blunt ends and often introduces small insertions and deletions along the way. HDR uses homologous...overhang. This will serve as both a substrate for proteins required for strand invasion and a primer for ...template to create mutations HDR donors can contain insertable sequences or specific mutations, along with homologous...target sequence to be modified. In general, the insertion sites of the modification should be very close...

  9. March for Science

    Type
    Blog Post
    ...apolitical process for seeking knowledge. The process begins with a testable hypothesis - an educated guess ...scientists then revise their hypotheses and the cycle begins again. No part of this process is inherently political...States government? Politics and science are intrinsically linked: governments exist in reality and science...Environmental Protection Agency, and the National Institutes of Health endanger scientists’ ability to uphold...consequences While frustration and anxiety have inspired people to rally for science, others are concerned... themselves can come to conclusions themselves instead of depending on scientists or politicians to interpret...lines (18). Feeling like an apple will fall up instead of down when dropped will not make it happen. In...

  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...transgene.The AAV encoding part A of the transgene contains a promoter and the 5’-fragment of the transgene...donor. The AAV encoding part B of the transgene contains a splice site acceptor followed by the 3’-end ...methods. The AAV encoding part A of the transgene contains the promoter, the 5’ half of the transgene, a ...homology. The AAV encoding part B of the transgene contains the region of homology, a splice acceptor sequence...help prevent the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a...directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site in the genome...stuffer DNA after the gRNA target site. Donor B contains: 400 bp homology arms to donor A,and “part B” ...
Showing: 281 - 290 of 1046 results