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We narrowed to 1,011 results for: CAL;

Showing: 561 - 580 of 1011 results

  1. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...in Plasmids 101, tags can help you determine localization of a protein of interest, purify it, or determine...major caveat - a tag may interfere with protein localization and/or function, so each tagged protein must...proteins found that 20% of tagged proteins did not localize similarly to their untagged counterparts. These...fluorescent-protein tagging show high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013...

  2. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands,” ...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their..., the brain is protected from stress-induced biochemical changes that could otherwise lead to depression...

  3. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions for defining the problem... training too many scientists and some propose radical changes to the way we view the graduate school ...University founded a successful consulting company called The Balsa Group. University of Pennsylvania has...

  4. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford University...as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron resolution...I have worked many hours in a dark room with a confocal microscope, yet I was once again fascinated by...them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...excellent Technical knowledge and creativity Become a “thought leader,” develop a technical “niche,” develop...FierceBiotech to “learn the business” Attend events with local networking and trade organizations Another excellent... be found on the UK Career Site for Researchers called Vitae. Vitae recently published a career development...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...pride in facilitating the exchange of not just the physical samples, but also the legal documentation that... Addgene has adopted the terms of the Uniform Biological Material Transfer Agreement (UBMTA) for its material... may not use the material in human subjects or clinical trials Acknowledgement in recipient’s future...have opted into our eMTA system can even sign electronically or choose to auto-approve MTAs. Whatever your...

  7. Let There Be LITE Plasmids

    Type
    Blog Post
    .... All of this requires genes to be regulated dynamically.” To understand how that works in memory formation...they could package LITEs in viral vectors and genetically target them to specific cell populations. They... the genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids...with LITE? Reference: Silvana Konermann et al. "Optical control of mammalian endogenous transcription and...

  8. Reflections and Looking Ahead

    Type
    Blog Post
    ...Antibody characterization is critical to enhance reproducibility in biomedical research. eLife, 13, e100211...launched AAV Packaged on Request, a service that dramatically expands and simplifies your viral vector options..., K., Alshafie, W., Baker, M. R., Ball, A. R., Callahan, D., Cooper, J. A., Crosby, K., Harvey, K. J.,...

  9. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ... therapies,” said Kris Wood of Duke University Medical Center. “While we do not yet have a comprehensive...resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully, the researchers...generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and it took them... he said. “It could [be used to screen for] morphological features, migration, or survival in different...

  10. Science as Art

    Type
    Blog Post
    ...while - a tool to expand perception, which is ironically what science is about. For me it is essential...living organism I study, to be able to see it all scales. Most modern biologists focus on one aspect alone... time, defend the thesis, etc. Photography is critically important in many fields of biology and I believe...appreciated by a wider audience for more than just its biological importance. More of Daniel's stunning work can...

  11. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...the lab with parties in the lab’s common areas, local pubs, and homes. The Church lab also toasts a newly... Improving your work-life balance Balancing the Scale: NSF's Career Life-Balance Initiative Can Scientists...

  12. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for...upcoming grant deadlines, and sync everything to your calendar. Ditch the paper To Do List and move to one that...reminders. These programs can be synced to your online calendar and can be accessed from your computer or smartphone... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...

  13. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long ...by direct transformation. A given insert will typically contain both a protein modification sequence and... not only track the abundance, movement, and localization of individual proteins, but also investigate... green, and red FPs that are codon optimized specifically for expression in yeast. These tagging vectors...

  14. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...scientists are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It...shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and...feedback in response to norepinephrine release, and clinically-used drugs targeting these receptors cannot be...nLightR, both generating a measurable signal after electrical stimulation with a single pulse, while nLightR...Cui, G., Lin, D., Du, J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific...

  16. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging...not recommended reagents) along with information critical for the performance of a reagent, e.g., antigen... (2022). IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse...Germain, R. N. (2020). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial...

  17. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ... may not even get a solution to the problem you called about. Next, you try an email, given that this ...that can help you through any search, ordering, technical, or quality control issues. Improving customer...chance to stop what you’re doing and make a phone call or write out an email. You need help right at the...support to address this exact problem, and it’s called “self-service.” The idea here is to build features...

  18. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...successfully resolved loci with physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances...makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has many applications, the...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart...

  19. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...choices.” She points out that a PhD teaches analytical and critical thinking skills that are applicable to ...

  20. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... (PA) near-infrared fluorescent proteins (FPs), called PAiRFP1 and PAiRFP2, to the in vivo imaging toolbox...of specific wavelength and intensity allows for optical labeling and tracking of proteins, organelles, ...resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter with fluorescence... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...
Showing: 561 - 580 of 1011 results