...common antibody applications.  Thanks for watching, and we hope you found the video radd-ically helpful!  ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place...

...the details. Chapter 3 - Answering interesting biological questions using microscopy. Chpater 4 - Research...speeds science. Chapter 8 - The importance of communicating research findings through social media and other...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him...

...on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders DNA ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

...with Addgene Board Member, depositor, and Harvard Medical School researcher Connie Cepko. Listen to the podcast... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...editing technology, Cpf1. Written by our Science Communications Intern (and soon to be Addgene Scientist!) ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...about how FRET can be used to study biological phenomena and about how fluorophores other than fluorescent...

...community into developing regions like South America and Africa, but we need to expand our efforts to find...challenges. However, these same researchers face significant barriers to scientific discovery and career development...

...important immune cell type, in more complex and physiological microenvironments. These screens have primarily...molecular identifiers, which can improve the statistical power of in vivo screens. Figure 1: Using...Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in improving CD8+ T cell...

...finishing up their time in the PhD Program in Biological and Biomedical Sciences at Harvard University. Ben and...Writing 5 Reasons to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc ...

...alternatives for some of the harmful chemicals in your lab. These chemicals are not only better for the environment... resources about this, and even has a list of biological samples that are stored long term at -70 °C. ...

...genome project and constructing ever more complicated biological devices. Chapter 6: - Making research go...foundational research, training, startups, and real applications of SynBio. Chapter 3: Synthetic biology as the...

... It's been an honor to work with such a group dedicated to providing the structure and know-how to integrate...Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering Committee...A grant from the National Institute of General Medical Sciences (NIGMS) will support the development and...

...Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions for defining the problem... training too many scientists and some propose radical changes to the way we view the graduate school ...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...at target sites. Read the paper in Nature Communications Find the Cas12b plasmids  Plant base editing...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...

...conference, you could be honing your science communications skills and networking. And on social media,...one “safe” project that you know you can get a publication with and one “shoot for the moon” project that... the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator Networking “Many,...

...virtually. 6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider by Jennifer Tsang The...The nanodrop vs. spec debate dominates DNA quantification discussions, but what about the other methods... with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel To complement ...

...sequence in live cells. This technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to...scientists. You can find all of our August posts in chronological order here: Site Directed Mutagenesis by PCR...Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing...

...2 trophies over the years, which is either an indication of the stiff competition or says something about...building activity that is easy to organize. We’ll dedicate a post to these competitions in the future, and...establish, with the goal of fostering better communication between labs. Scientists are often willing to...

...Annotation of genes in immune cells typically involves the creation of germline knockout mice, which ...Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in improving CD8+ T cell...screening of genes in the immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... helps you reach your goals Listens well and communicates clearly and openly Gives honest feedback without...lab. If only every science trainee had such a dedicated person guiding the start of their career. “I want...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus...methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you have a new...

...important. It needs to express value, clearly communicate exactly what a site offers to their users, and....  With these three main values we set out to radically redesign our homepage to reflect this message....hug items closer and do so more animatedly. To communicate that sense of community, Blugene will regularly...

...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

...control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs throughout...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted...require a permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics...

...let curiosity guide your intellectual journey. Practical approaches One approach to achieve this goal ...chemistry, even if your primary focus lies within a biological lab. Engaging in such seminars exposes you to...ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking...

...There must be some way to use LEGOs, which are practically universally loved (except for those rare times...children who have no preconceived obstacles, optimistically grasp at limitless potential, and who sometimes...Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Resources on ...

...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,... of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587...

...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

...reproducibility and an inability to improve upon these critical research tools. Addgene is excited to announce...in neuroscience research today, but there is a critical unmet need for an open-access, molecularly defined...such as plasmids and viral vectors. However, it typically takes a few years for a new project to reach a...