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We narrowed to 954 results for: App;

Showing: 561 - 580 of 954 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ... its reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of...the same time from two different plasmids this approach becomes problematic as it reduces the probability... Cell Types for Imaging and Long-Range Circuit Mapping.” Journal of Neuroscience 28(28): 7025–30. PubMed...

  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...institutions, or institutions they're thinking about applying to, are doing - for example, your benefits may...get rid of postdocs; that current postdoctoral appointments may shorten in length; that postdoc salaries...trainee workforce has clearly not been a sustainable approach to biomedical research in the U.S. and postdoctoral...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...especially with industry. Today we also have the appropriate tools to verify our cells lines. Even if you ...the results are reliable. With this two-pronged approach, the many mistakes of the past can be prevented...including GSK, AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...Scarless Modification of Proteobacterial Genomes." Applied and environmental microbiology (2019): AEM-00990...chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015): 5103-5114. PubMed...

  5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...study its function is one of the most popular applications of fluorescent proteins. These fusion proteins...targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay is quantitative and...linker sequence short In the early days of GFP applications, many were concerned with steric hindrance by...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...enjoy a warm meal on a winter day, you should be happy to learn that many DNA-based reagents are named ...aptamer soup by exploring the development and applications of fluorescent RNA aptamers in Addgene’s collection...fluorescence when bound to DFHBI despite being approximately half the length (Warner et al., 2014); its length...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... barrier of the endothelium, but they could be applied to study other actin-dependent processes in cells...) and to introduce unnatural head groups for applications like fluorescent labeling via click chemistry...constructs are great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks...

  8. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... explain how to verify that your cells were appropriately edited for your desired mutation - insertion...frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, 50% are -/+, and...sequencing confirmation. All knock-ins A method applicable to knock-ins both large and small is a software...

  9. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...can be engineered to bind specific RNA bases. By swapping out these different modular repeat segments, you...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

  10. Pouring LB Agar Plates

    Type
    Protocol
    ...concentration disolved in the appropriate liquid solvent. See table to the right for appropriate antibiotic concentrations...Strains Grow Assuming the appropriate strains were streaked on the appropriate plates, then if both strains... LB-agar powder you’ve measured out into an appropriately sized bottle for autoclaving. We make 400 mL...autoclave. Be sure to check the literature for an appropriate sterilization technique if you are working with... wipe down with a paper towel. Count out the appropriate number of plates and stack them on your lab bench...of molten gel mix, a tube rack containing the appropriate antibiotics, and a section for active pouring...antibiotic. Incubate both plates overnight at the appropriate growth temperature and check for growth. See ...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A....that they could reprogram the genomic target by swapping the homology arms for new targets. And, importantly... is scarless, unlike previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety... publication at Addgene. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy...Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based approach for targeted DNA demethylation. Cell Discovery...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...tools Once you’ve selected the class of tool appropriate for your experiment, you’ll need to find a specific...Yizhar, O., & Deisseroth, K. (2012). Principles for applying optogenetic tools derived from direct comparative...

  14. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...the coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, ...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi...cumate-inducible gene expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi...expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res...

  16. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...have been increasingly popular in fundamental and applied research since their first use in the early 90’... at transferring the gene of interest to the appropriate cells, it was obvious that its safety was a disadvantage...either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express only the ...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...cross-team guy” at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that...identify as one.  But some time around 10th grade, I snapped out of the phase of my life that had me listening...you’re part of a team - it will just make you a happier person. What does a good team need? Besides that...

  18. Protocol - Bacterial Transformation

    Type
    Protocol
    ... thaw on ice (approximately 20-30 mins). Remove agar plates (containing the appropriate antibiotic ) from...spreading device Reagents LB agar plate (with appropriate antibiotic) LB or SOC media Competent cells DNA...Pro-Tip Transformation efficiencies will be approximately 10-fold lower for ligation of inserts to vectors...transformation onto a 10 cm LB agar plate containing the appropriate antibiotic. Pro-Tips We recommend that you plate... take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane permeability... to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's instructions...

  19. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...your convenience. See “warranty information” in appendix. Table of Contents A. pLKO.1-TRC Cloning Vector...References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence of pLKO.1 TRC-Cloning Vector I.2 ...GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001 OPTI-MEM® serum-free ...protamine sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...overnight. Day 2: b. The target cells should be approximately 80-90% confluent. c. Dilute puromycin in the...increments. d. Label plates from 1-10 and add appropriate puromycin-containing media to cells. Days 3+:...overnight. Day 2: b. Target cells should be approximately 70% confluent. Change to fresh culture media...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition editors. They...GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing. Instead of fusing ...abasic site. In this post, it refers to the same approach as a CGBE. BER Base Excision Repair A cellular...
Showing: 561 - 580 of 954 results