We narrowed to 658 results for: EPO;
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TypeBlog Post...proteins. Here, we’ll highlight a few recently-deposited optogenetic tools to control the cytoskeleton ...
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Filming Science Videos in the Age of Social Distancing
TypeBlog Post...repurposed for a different shoot to create the How to Deposit Plasmids video. Shot without anyone else in the... -
Transferable Skills Guide: Time Management
TypeBlog Post... give it a final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post... the Patron Lab. As with all plasmids in the repository, we highly recommend reading the associated publications... -
Site Directed Mutagenesis by PCR
TypeBlog Post...regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional analysis of proteins... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...co-expression of a suppressor tRNA (SupD) instead of the SepOTSl can encode a non-phosphorylated serine at the TAG... -
Evolution of Lab Techniques
TypeBlog Post...already commercially available although they are reported to have high error rates (10). It is projected... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...drop down menu of common cloning options in the deposit process in 2014 because of its gain in popularity... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...(the current median postdoc starting salary as reported by Paula Stephan is $40,000) Increase Knowledge... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct... -
Plant Plasmids and Resources
TypeCollection...Proteins Other Resources Background Addgene’s repository contains many plasmid tools that were specifically...tools). Support open science in plant biology - deposit plant plasmids with Addgene! CRISPR Please refer... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post... Interest Into a Variety of Expression Vectors Deposit Your Microbiology Plasmids ... -
Viral Production
TypeCollection...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a... -
Neurodegeneration Research Collection
TypeCollection... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell... -
Important Considerations When Using AAVs
TypeBlog Post... complexes to study neural function. Often the reporter proteins associated with these complexes are so... -
Finding Your Science Policy Path
TypeBlog Post...science policy skills in graduate school via this report from the Scientific Citizenship Initiative. ...