We narrowed to 707 results for: ESC;

...of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...

...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...

...excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction...

...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

...free of contamination and match their original description. The oldest, largest, and most diverse of these...

...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

...don't see protein overexpression? The strains described above should generate sufficient expression levels...

...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...