... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...

...genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA Transposons and the Evolution...

...3rd year graduate student in the lab of Prof. Francesco Paesani at University of California San Diego....

...PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural...

...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...

...Fact! Even though the Cas9:RNA complex is well described in the closed conformation, recent electron microscopy...

...’s first demonstration of prime editing used to rescue a genetic disease phenotype in an animal model,...

... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...

...Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty...with N and C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with...cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein...yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein...pRSETa mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: ...blot. The plasmids in this collection contain fluorescent protein tags whose localization you can monitor...Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids Blue/UV , Cyan ...

... DULIP ( DU al L uminescence-based C o- I mmunoprecipitation) plasmids : Luminescence-based protein-protein...Double‐Readout Lu minescence‐based T wo‐ Hy brid) : Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation...for assays of transcriptional regulation and bioluminescent reporters. Plasmid...Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights...Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging...delivered intracellularly in order to measure luminescence. Gaussia luciferase is secreted and more stable...