We narrowed to 706 results for: ESC;

1. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...

2. An Inside Look into Addgene's Dev Team

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   Blog Post

   ... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

3. Plasmids 101: Gateway Cloning

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   Blog Post

   ...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

4. Changing Labor Laws Bring Increased Postdoc Wages

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   Blog Post

   ...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

5. CRISPR Activation: A Practical Guide

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   Blog Post

   ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

6. The Challenges of Cell Culture

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   Blog Post

   ...free of contamination and match their original description. The oldest, largest, and most diverse of these...

7. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

8. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...don't see protein overexpression? The strains described above should generate sufficient expression levels...

9. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

   ...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

10. CRISPR 101: Validating Your Genome Edit

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    Blog Post

    ...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

11. Transferable Skills Guide: Conflict Resolution

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    Blog Post

    ...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

12. The Advances Behind The World's First Personalized CRISPR Treatment

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    Blog Post

    ...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    ...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

14. Hot Plasmids: Summer 2024

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    Blog Post

    ... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

15. Plasmids 101: Biotinylation

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    Blog Post

    ...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

17. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

18. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

19. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

20. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...