We narrowed to 703 results for: ESC;

...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...

... on a new feature. We start with a story that describes the feature and what the criteria is for accepting...

...for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases reprogramming...

..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...

...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

...don't see protein overexpression? The strains described above should generate sufficient expression levels...

...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...