We narrowed to 639 results for: ESP

1. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   Blog Post

   ...colleagues who read it with me, the consistent response from scientist and non-scientist alike was that...

2. DNA Purification Without a Kit

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   Blog Post

   ....1371/journal.pone.0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification...

3. Simplify Cloning with in vivo Assembly

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   ...vector with restriction enzymes and adding the corresponding homologous sequences to the insert by PCR. ...

4. Uncovering Ancestral Bias in CRISPR Research

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   Blog Post

   ...variability.   Moving forward It took a five-team response to address this issue, but it still points to ...

5. MXS Chaining

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   Blog Post

   ...expression and detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining...

6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...mono-methylation, tri-methylation, and aceylation, respectively (these are chemical modifications, or functional...

7. Lentiviral Vector Uses and Overview

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   Blog Post

   ...amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise that researchers...

8. Plasmids 101: Colony PCR

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   ...you used for cloning. This type of control is especially important for insert-specific primers. If your...

9. CRISPR Antimicrobials

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   Blog Post

   ...beta-lactam resistant population to the antibiotic, despite this resistance gene being encoded by a high-copy...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is...

11. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...

12. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    Blog Post

    ...holding the tubes and use this ID to name the corresponding Excel sheet.3. Be specific  - When assigning...

13. A Career in Grants: How To Become a Grant Professional

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    Blog Post

    ...consultant to faculty writing research grants, especially in the sciences and by extension to scientifically...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...

15. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...risk. Biosafety should always be considered with respect to the specific experiments being performed and...

16. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post

    ...letter following the enhancer ID (h, m, or q, respectively). These sequences were then cloned into an AAV...

17. QC Sequencing Technologies at Addgene

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    ...scaffold to map the reads, which boosts ‌accuracy, especially in repeated regions. If this doesn’t resolve ...

18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ...easy-to-understand guided manner. These videos can be especially useful when designing new primers to clone your...

19. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...cerevisiae and E. coli. Auxotrophic selection is especially common in yeast experiments because yeast tend...

20. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...recognized or leaky in Agrobacterium. This is especially problematic with reporter genes as you might ...