We narrowed to 637 results for: ESP

1. DNA Purification Without a Kit

   Type

   Blog Post

   ....1371/journal.pone.0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification...

2. MXS Chaining

   Type

   Blog Post

   ...expression and detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining...

3. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   ...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   ...mono-methylation, tri-methylation, and aceylation, respectively (these are chemical modifications, or functional...

5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   ...you to lose soluble proteins from the sample, especially if left on for too long or used at too high a...

6. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ...vector with restriction enzymes and adding the corresponding homologous sequences to the insert by PCR. ...

7. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   ...amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise that researchers...

8. Plasmids 101: Colony PCR

   Type

   Blog Post

   ...you used for cloning. This type of control is especially important for insert-specific primers. If your...

9. CRISPR Antimicrobials

   Type

   Blog Post

   ...beta-lactam resistant population to the antibiotic, despite this resistance gene being encoded by a high-copy...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    ...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is...

11. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    ...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...

12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...holding the tubes and use this ID to name the corresponding Excel sheet.3. Be specific  - When assigning...

13. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...consultant to faculty writing research grants, especially in the sciences and by extension to scientifically...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    ... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...

15. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    ...risk. Biosafety should always be considered with respect to the specific experiments being performed and...

16. Hot Plasmids - November 2023

    Type

    Blog Post

    ...-iPAK4 expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example...

17. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    ...scaffold to map the reads, which boosts ‌accuracy, especially in repeated regions. If this doesn’t resolve ...

18. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    ...easy-to-understand guided manner. These videos can be especially useful when designing new primers to clone your...

19. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    ...cerevisiae and E. coli. Auxotrophic selection is especially common in yeast experiments because yeast tend...

20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    ...sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth...