Addgene

We narrowed to 628 results for: ESP

Showing: 561 - 580 of 628 results

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Lentiviral Vector Uses and Overview

Type

Blog Post

...amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise that researchers...
Plasmids 101: Colony PCR

Type

Blog Post

...you used for cloning. This type of control is especially important for insert-specific primers. If your...
CRISPR Antimicrobials

Type

Blog Post

...beta-lactam resistant population to the antibiotic, despite this resistance gene being encoded by a high-copy...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...holding the tubes and use this ID to name the corresponding Excel sheet.3. Be specific - When assigning...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...consultant to faculty writing research grants, especially in the sciences and by extension to scientifically...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...risk. Biosafety should always be considered with respect to the specific experiments being performed and...
Hot Plasmids - November 2023

Type

Blog Post

...-iPAK4 expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example...
Analyzing CRISPR Editing Results with ICE from Synthego

Type

Blog Post

...sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth...
To Codon Optimize or Not: That is the Question

Type

Blog Post

...’ end of the tRNA binds the amino acid that corresponds to the anticodon sequence. That means there are...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...SpRY, can target NGN PAMs and NRN and NYN PAMs, respectively (Walton et al., 2020). Characterization of Cas9...
Save Time with Transient Plant Leaf Transformations

Type

Blog Post

...recognized or leaky in Agrobacterium. This is especially problematic with reporter genes as you might ...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...neurotrophic factor (BDNF). Bdnf is expressed early in response to neuronal activity (hence, “immediate-early”...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...questions can derail your talk. You need to balance respectfully answering questions while steering the conversation...
Lab to Office Culture Shock

Type

Blog Post

... to which she was applying. I probably did not respond to her question as well then as I would now. I ...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

...of pTargetF into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...References del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...chunks, we have the flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another...

Showing: 561 - 580 of 628 results

Results per page:

Lentiviral Vector Uses and Overview

Plasmids 101: Colony PCR

CRISPR Antimicrobials

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Tips for Screening with Yeast Two Hybrid Systems

Tips from the Repository Trenches: Using Barcodes to Track Samples

A Career in Grants: How To Become a Grant Professional

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Your Lentiviral Plasmid FAQs Answered

Hot Plasmids - November 2023

Analyzing CRISPR Editing Results with ICE from Synthego

To Codon Optimize or Not: That is the Question

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Save Time with Transient Plant Leaf Transformations

New Tools Enable CRISPRa for Neuroscience Applications

Designing Your Chalk Talk for the Academic Job Interview

Lab to Office Culture Shock

CRISPR-based Genome Editing Systems For Editing in E. coli

Plasmids 101: Plasmid Incompatibility

An Inside Look into Addgene's Dev Team

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