...unpredictable loud noises and flashing lights - that sent average, control mice into depression. In case you were... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...between the muscle and brain, which may have therapeutic implications, say Hyo Youl Moon and Henriette...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...Pettersson AT, Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016...

...for general audiences. So far, we are beginning to make an impact on society and science literacy. We ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...

...become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center...that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene. “We called... cancer therapy...

...that Addgene is officially filed, recognized and operated as a nonprofit under the United States Internal...fulfilling our mission. “Addgene’s nonprofit mission: Accelerate research and discovery by improving access to...in that we are able to fulfill our mission by generating revenue through distribution of research materials...nonprofit" is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution...nonprofit, open science and Addgene in our mission to accelerate research with easier sharing and collaboration...

...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...

...saves time, knowledge and makes your insights discoverable. Watch this quick video to learn how ReFigure...How much time do you spend reading scientific literature? Very often the data of interest are in 1 or ...preclinical and clinical data about a disease or therapeutic Side-by-side comparison of the same experiment...save analyses with the relevant figures from a literature review. No more hunting through a whole folder...reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology...

...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques, and...Site (MCS) Short segment of DNA which contains several restriction sites allowing for the easy insertion...later work.     How do scientists use plasmids? Generally, scientists use plasmids to manipulate gene expression...Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular... viruses that can be used in research or for therapeutics Addgene has compiled various educational resources...

...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...the receptors are named after the isotypes they interact with, so IgG isotypes bind to Fcγ receptors; IgE..., ADCC can be induced through a very similar interaction between IgE antibodies and eosinophils, where... induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies ...phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI:10.1126/sciadv.abl9171...

..., functions at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles.  ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible gene...Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image from Sun...successfully targeted cells that can be used to generate organoid cultures with a modification of interest...

...by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged... exhaustive refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16...treated with the luminescent enzyme substrate coelenterazine, followed by the addition of the endogenous...

...that in January. This involves going in front of a camera and kind of walking them through the steps of, ... heart to look at the inside. We do a lot of interactives and videos, as well, to supplement the hands-on... Caitlin also collaborates with the sales and operations departments at eScience Labs. This can involve...eScience warehouse is onsite, Caitlin can visit the operations team to make sure “that everything that’s going...facilitator between her team, the sales department, and operations department with the ultimate goal of determining...

...vivo (try pcDNA3-EGFP). If you can get away with generating transiently transfected cells, you will not need...that you will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great...binding or folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your...microscope, we offer a collection of vectors carrying several fusion proteins. Pick your promoter, selection ...promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms...

...peptides consist of as little as 8 or as many as several hundred amino acids, depending on the tag, and ...charged amino acids. FLAG tag has been used with moderate to high success rates across all applications....including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide... has two negatively charged amino acids for an overall negative charge. This tag has been used successfully...Additional resources on Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody protocols...

...chromosomes are represented by two to three copies, but several have four. No Y chromosome is present in this line... line, making it genetically female. HEKs are generally cultured as an adherent monolayer, though derivatives...plasmids with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ ...the way up to the production of clinical viral therapies. Protein production  HEKs are extremely easy ... and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells can catch...

...pluripotent state. Though a valuable tool for regenerative medicine, direct reprogramming is an inefficient...demultiplex the scRNA-seq data and build multi-generational cell lineage trees. CellTags were detected over...MettI7a1 encodes an uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α... at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...methods for delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers...

...responsible for a phenotype can be identified by next-generation sequencing of the genomic DNA. However, some ...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter...approach to expand our understanding of ApoB-LPs and accelerate the discovery of additional genes and small-molecule...tool should serve as both a powerful tool for generating complex regulatory systems and further the study...nematode Caenorhabditis elegans. They have found several voltage biosensors and conditions that can be used...

...zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger... for Synaptic Imaging However, the first two generations of iGluSnFR had limitations: they were not optimal...extrasynaptic glutamate release. Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement...not neurons). iGluSnFR3 is also available in several ready-to-use viral vectors.    References and ...

...SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence and ultimately its degradation... plant protein TIR1 in non-plant cells, which interacts with the cells’ endogenous ubiquitin ligase, the...with mAID. Using this method, the lab was able to generate conditional alleles of a few essential proteins...attempted to engineer loops to create genomic interactions that wouldn’t normally occur. The synthetic ...enhancer. What makes LADL unique from previous iterations is the addition of CRY2, another Arabidoposis...

...encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been developed, such as... and fluorescence based reporters however have several limitations. First, FRET signals are often difficult...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...Cabantous, Stéphanie, et al. "A new protein-protein interaction sensor based on tripartite split-GFP association...

...successful mutation of your favourite gene, there are several different methods available. Thankfully, there ...validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the gold standard...emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will...desired genome edit. Since exploitation of NHEJ to generate translational reading frame-altering indels for...validation methods As mentioned, these assays have several major advantages over the commonly employed mismatch...