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We narrowed to 111 results for: Eras

Showing: 1 - 20 of 111 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...respectively), Cypridina luciferase , and Luciola luciferase . Each type of luciferase has advantages and disadvantages...CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return... Plasmid Collections Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent ...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often...Luc2. Other luciferases in our collection include the red and green Click-beetle luciferases ( CBRluc and...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative diseases are a group of disorders... the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases include...most neurodegenerative diseases are only partly understood, and there are no effective therapies to prevent...available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred...Addgene that may be useful for neurodegeneration research. Neurodegeneration... Neurodegeneration Research Collection Addgene ...plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection ...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection ... 54155 mEmerald-MAPTau-N-10 MAPT mEmerald CMV Parkinson's, FTD Michael Davidson 54226 mEmerald-Peripherin-C...mEmerald-Peripherin-C-14 PRPH mEmerald CMV ALS Michael Davidson 54227 mEmerald-Peripherin-N-18 PRPH mEmerald CMV ALS Michael... on genes and associated neurodegenerative diseases was curated from several sources (Chan Zuckerberg ...Parkinson's John Chodera 79712 E2AK2_HUMAN_D0 EIF2AK2 His, TEV T7 VWM disease John Chodera 82285 pDONR223...Lippincott-Schwartz 164210 pLEX-EF1a ANXA11-mEmerald ANXA11 mEmerald EF1a ALS Jennifer Lippincott-Schwartz ...Lippincott-Schwartz 164211 pLEX-EF1a ANXA11-mEmerald D40G ANXA11 mEmerald EF1a ALS Jennifer Lippincott-Schwartz 164212 pLEX-EF1a...

  4. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here....Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative Disease Initiative...Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...Initiative (iNDI) project aims to generate and characterize single nucleotide variant lines in an isogenic cellular...Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution efforts. Plasmids...NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases with genomic engineering: A new stem...

  5. CRISPR References and Information

    Type
    Collection
    ...predictor. Developed by the Jin-Soo Kim Lab . CRISPR-ERA: CRISPR -mediated e diting, r epression, and a ctivation... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...species. Developed by the Kate O'Connor-Giles Lab . General Resources CasPEDIA (Link opens in a new window)...entries describing enzyme activity, experimental considerations, and more. CasPEDIA is a community resource...Link opens in a new window) CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9...

  6. Open Enzyme Collection

    Type
    Collection
    ... DNA Polymerase I 165533 pOpen-TthpolA Tth DNA polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified... Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other Enzymes... of molecular biology, including DNA polymerases, RNA polymerases, DNA ligases, reverse transcriptases... DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 DNA polymerase 165501.... Heat-Stable DNA Polymerase 165502 pOpen-9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating ...pOpen-K12polLF DNA Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (...

  7. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...channel Mouse Chimera: Mouse/Rat rat IgG2a 222182 RGS14 [N133/21R-rat IgG2a] RGS14 Rat Chimera: Mouse/Rat...receptor Rat Chimera: Mouse/Rat rat IgG2a 222188 GFP [N86/8R-rat IgG2a] GFP Aequorea victoria Chimera: Mouse... to the research community. Protocols for the generation R-mAbs plasmids, production of R-mAbs in transfected...researchers will be able to easily and inexpensively generate R-mAbs by transiently transfecting the plasmids...channel [K14/16R-rat IgG2a] Kv1.2 K+ channel Human Chimera: Mouse/Rat rat IgG2a 222178 CASPR/Neurexin IV [... [K65/35R-rat IgG2a] CASPR/Neurexin IV Rat Chimera: Mouse/Rat rat IgG2a 222179 GluA2/GluR2 glutamate receptor...-rat IgG2a] GluA2/GluR2 glutamate receptor Rat Chimera: Mouse/Rat rat IgG2a 222180 Slo1/BKAlpha maxi-K...

  8. CRISPR Guide

    Type
    Collection
    ...using dual nickases to generate a knockout or gene edit; or using Cas9 to generate large genomic deletions...proteins. Large deletions generated by Cas3 have fewer off-target alterations, such as small mutations ...nucleases (TALENs) required scientists to design and generate a new nuclease pair for every genomic target. ..., and precisely edit DNA and RNA. The ease of generating gRNAs makes CRISPR one of the most scalable genome...and some of the ways the CRISPR components are leveraged to achieve specific goals, such as targeting multiple...depends on which Cas protein you use, and there are several to choose from. Many variations of Cas9 exist, ...will form a ribonucleoprotein complex through interactions between the gRNA scaffold and surface-exposed...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ...collection of plasmids for the generation and study of stem cells, from iPSC generation to directed differentiation...source for therapeutic use. Since 2006, scientists have developed many approaches to generate iPSCs. The... post Delivery Methods for Generating iPSCs provides an overview of several different reprogramming methods...Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Stem... Stem Cell Research Plasmids Stem cells are proliferative, unspecialized cells with the capacity to differentiate...pluripotent or multipotent, based on their ability to generate different cell lineages. Pluripotent stem cells... for drug development, disease modeling, and regenerative medicine. iPSCs, much like their ES cell cousins...

  10. Plan Your Experiment

    Type
    Collection
    ...want to: Generate complete and permanent loss of gene expression or function (knockout)? Generate a specific... for different organisms. This page provides a general framework to get you started using CRISPR in your...genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment. Created with ...Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt...verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations or...efficiency compared to NHEJ knockout Cut Nick Base Edit Generate specific base pair edits without double-strand...proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions, deletions, and point mutations...

  11. Bacterial Expression Systems

    Type
    Collection
    ...Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein Interactions Return ...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids...Protein Purification Protein Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome...function, structure, and activity of a protein it is generally necessary to purify it. Specific bacterial strains...Fluorescent Protein Timers Return to top Protein Interactions Förster resonance energy transfer (FRET) and...have been widely used to study protein–protein interactions using fluorescence microscopy. FRET relies on...

  12. Promega Plasmid Collection

    Type
    Collection
    ...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter... two proteins interact, the subunits come together to form an active enzyme and generate a bright luminescent...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...solutions that help advance science while reducing operational complexity. This unique collection contains plasmids...experimental workflows. The collection contains luciferase reporter vectors for sensitive gene expression..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other...

  13. Antibody Guide

    Type
    Collection
    ...signaling molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP ...with several suitable antibodies; if they all meet your criteria, you may either purchase several to compare...epitopes on one antigen. Monoclonal antibodies are generated from cultured B cells, where each culture should...variability. Other Affinity Reagents There are several alternatives to antibodies, ranging from antibody... aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles. Here are a few tips for storing...a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...Applications Antibody-based applications can be generally classed into four categories: Quantification -...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...sequence and eliminating interactions with endogenous factors. Experimental Considerations Expression System ...relative ratio. Different generations of transactivators and promoters are generally cross-compatible. Your...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...Tetracycline Off Tetracycline On Experimental Considerations Tet Plasmids Inducible and repressible promoters... the tetracycline repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA...other tet- or dox-dependent promoters (sometimes generally called Ptet) have been designed by combining tet...than simply removing the repressor. 2nd and 3rd Generation Systems Subsequent efforts have improved the ...

  15. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein. Protein Phosphorylation...phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing recombinant...further details in Mohler et al. (2023) and find general tips on phosphoprotein expression in rEcoli XpS...Barber et al., 2018). This phosphosite library was generated in a single mixed pool by expressing and purifying...assessments. The Mode #1 phosphosite library can be generated with either phosphoserine using SepOTSλ (Plasmid...screening phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library...

  16. Lentivirus Plasmids

    Type
    Collection
    ...used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these...potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have ...information about: Lentiviral components 2nd versus 3rd generation lentiviral systems Viral production Frequently...produce an infectious viral particle (a virion) are generally divided among multiple plasmids. Some plasmids... Packaging Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging...Packaging Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding...

  17. Rett Syndrome

    Type
    Collection
    ...curated literature information (Link opens in a new window) OMIM MECP2 - MECP2 curated literature information...MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to...contrast to the sustained loss of skills in neurodegenerative conditions, the regressive period in Rett ...locally or globally. The MECP2 protein contains several functional domains, including: the N - t erminal...acid and Thyroid hormone receptor (NCoR/SMRT) interacting domain (NID) a nuclear localization signal (not...the MECP2 protein in mouse and rat models have accelerated our understanding of Rett syndrome at the molecular...They are also an important tool for developing therapeutic strategies to treat Rett syndrome and restore...

  18. Brain Initiative Collection

    Type
    Collection
    ...the revolutionary dynamic picture of the brain generated through this effort will provide novel ways to...Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111068-AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2...promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2...promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.1...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2... vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...poorly understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety...the live virus. Alternatively, it is possible to generate pseudovirus, where the structural and functional...spike protein is inefficient for pseudotyping; several modifications may enhance infectious particle production...lists expression plasmids that are suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses...

  20. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact...Vladislav Verkhusha 79990 pKeratin-miRFP703 Intermediate Filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha...mCherry Alpha Yap 54219 mEmerald-Paxillin-22 Focal Adhesions paxillin mEmerald* Michael Davidson 15233...GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Michael Davidson 79997...EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc....Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal Junctions Secretory Pathway Background...structures as these well-characterized proteins. General Structures The cell contains a variety of organelles...
Showing: 1 - 20 of 111 results