...types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc...and NanoLuc® Luciferase (Nluc). Luc2 is the second-generation version of Firefly luciferase that has been... Plasmid Collections Luciferase Plasmids Luciferase Plasmid Collection You may also like...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent ...mammalian systems. Other luciferases in our collection include Gaussia luciferase (Gluc) and the red and...green Click-beetle luciferases (CBRluc and CBGluc, respectively). Like Firefly luciferase, Click-beetle red...type of luciferase has advantages and disadvantages depending on the application. Firefly luciferase is considerably...

...Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative diseases are a group of disorders... the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases include...most neurodegenerative diseases are only partly understood, and there are no effective therapies to prevent...available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred...Addgene that may be useful for neurodegeneration research. Neurodegeneration... Neurodegeneration Research Collection Addgene ...plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection ...

... Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection ... 54155 mEmerald-MAPTau-N-10 MAPT mEmerald CMV Parkinson's, FTD Michael Davidson 54226 mEmerald-Peripherin-C...mEmerald-Peripherin-C-14 PRPH mEmerald CMV ALS Michael Davidson 54227 mEmerald-Peripherin-N-18 PRPH mEmerald CMV ALS Michael... on genes and associated neurodegenerative diseases was curated from several sources (Chan Zuckerberg ...Parkinson's John Chodera 79712 E2AK2_HUMAN_D0 EIF2AK2 His, TEV T7 VWM disease John Chodera 82285 pDONR223...Lippincott-Schwartz 164210 pLEX-EF1a ANXA11-mEmerald ANXA11 mEmerald EF1a ALS Jennifer Lippincott-Schwartz ...Lippincott-Schwartz 164211 pLEX-EF1a ANXA11-mEmerald D40G ANXA11 mEmerald EF1a ALS Jennifer Lippincott-Schwartz 164212 pLEX-EF1a...

...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here....Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative Disease Initiative...Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...Initiative (iNDI) project aims to generate and characterize single nucleotide variant lines in an isogenic cellular...Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution efforts. Plasmids...NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases with genomic engineering: A new stem...

...Cas-OFFinder and Microhomology predictor. CRISPR-ERA: CRISPR -mediated e diting, r epression, and a ctivation...CRISPR Discussion Group (moderated by Feng Zhang lab): Forum/FAQ Drosophila: General Advice and Forum/FAQ ...entries describing enzyme activity, experimental considerations, and more. CasPEDIA is a community resource... KB Vosshall and Matthews CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9...

.... This is why they are called transferable skills. Why are transferable skills so important? It is important...   What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...much in my current role as Customer Support and Operations Manager at Addgene. In this role, I balance my...important to identify which transferable skills you possess because any potential employers, collaborators... examine not just technical skills, but also transferable skills, before offering someone their dream ...certainly matters in the job market, it is the transferable skills that create a full package and add an...for that ideal job. How can you identify your transferable skills? PLoS One recently published an article...

...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this... by highlighting that researchers learn many transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Negotiation. As a grad student...Hawaii?). So how can you approach improving this transferable skill? Negotiation as a zero sum game Some ...negotiation skills You can easily work on this transferable skill without any formal training or coursework...business development team, negotiation is a key transferable skill that can help you get more of what you...resources on the Addgene blog More from the transferable skills guide: Conflict Resolution Time management...

...Blog Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills...feel they lack formal training in a variety of transferable skills. At Addgene we're helping to fill this...both helping researchers recognize their MANY transferable skills learned while working in the lab and ...Today, we’re highlighting an oft-underlooked transferable skill: creativity. I’ve heard quite a few scientists..., it’s clear that creativity is absolutely a transferable skill developed during PhD training. Creativity...to do this and are still struggling with idea generation, try this thought exercise: Consider the issue...three-ring binder as a starting point, the ideas generated were hardly novel. When told to use a roller skate...

...iPSCs, the the ability to generate teratomas is the gold standard instead. Teratoma formation requires injecting...almost all available approaches for generating iPSCs. The generation of iPSCs is relatively simple in concept...delivery methods Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they ...transposase. A challenge of using PB to generate iPSCs is that genomic alterations can occur at the transposon insertion...has lower levels of efficiency at generating iPSCs, usually several orders of magnitude lower than retroviruses...making chimeras by injecting iPSCs into blastocysts and implanting them into female mice. Generating chimeric...Gene-delivery systems for iPS cell generation. Expert opinion on biological therapy, 10 2, 231-42. PubMed PMID...

...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ...innovative tools. Afterall, isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic...technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather dull 19kDa...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...Promega supports the Addgene mission statement: Accelerate research and discovery by improving access to... 19kDa Oplophorus luciferase by Promega’s Advanced Technologies group as well as evolution of the coelenterzine...

...work as well. Social interaction is a good thing Part of you needs to interact with other people, whether...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering... just Googled the phrase “People need social interaction” and received 104 million results (Here’s the...my bedroom. I realized I could and should be interacting more with other people, and becoming part of ...on the Addgene Blog Read other posts in the Transferable Skills Guide Management for Scientists: Managing...

...into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and...for gene (and cell) therapies. One may wonder why the first AAV-based gene therapy treatment for a genetic...forward with ocular gene therapy The pipeline of AAV-based ocular gene therapies is impressive: clinical...candidate therapies for these diseases. Riding on the early successes of retinal gene therapy, an ever-growing...further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the ...Stock of Retinal Gene Therapy: Looking Back and Moving Forward. Molecular Therapy Vol. 25 No 5. PubMed ...Muzyczka N., 2014. AAV-Mediated Gene Therapy for Research and Therapeutic Purposes. Annu Rev Virol. 2014 Nov...

...cloning of firefly luciferase was published in 1985, several studies utilized luciferase as a genetic reporter... plasmids expressing luciferase Luciferase reporter assay The typical luciferase-containing plasmid is...Well-established Biological sources of luciferase Although luciferase isolated from the firefly beetle (Photinus...with the substrate coelenterazine and is likely the second most commonly used luciferase. Other organisms...reniformis) 480 36 Coelenterazine None No Deep sea coceopod(Gaussia princeps) 480 20 Coelenterazine None Yes ...containing luciferase and the gene of interest permits translation of functional luciferase, until miRNA...promoter linked to luciferase. Activation of the pathway will result in luciferase expression and more...

...perfect transferable skill. Almost every other form of presenting will be easier than your average scientific...resources on the Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict ...of being a scientist and an incredibly useful transferable skill. Being able to stand up in front of an...biology class. A Cell paper. With six figures. Averaging ten panels each. (For this story, it is important... of data, my mind went completely blank. After several seconds of truly panicked silence, I opened my ...remember related to this paper and/or biology in general until I reached Fig. 6, Panel L, and hope nobody...remember to breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking...

... blog Management for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork...the right time. Like many of the posts in our Transferable Skills series, this post is not meant to be ... that you could strengthen. The right set of transferable skills, coupled with your technical skills, ...support each team “owns” is critical for day-to-day operations and keeping our customers happy, but inevitably...at that given time. I think about how previous iterations of those teams resolved similar issues in the...based on various levels of assertiveness and cooperation: avoiding, competing, accommodating, collaborating...

...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Problem-Solving. I don’t know ... careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined. In this ...because it helps me see problem solving as a generalizable process.  Step One: Identify the problem ...research to better understand it and come up with several hypotheses on the cause. To test the most likely...your life. This tactic is a good way to see how transferable these skills really are, while also practicing...

...slightly to Taq polymerase’s ideal temperature (70-75oC). At this temperature Taq polymerase can synthesize...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...Taq). Taq polymerase is thus a heat resistant enzyme that can withstand changes in temperature. Taq was...this temperature the primers can anneal to the single stranded DNA template strands. DNA polymerase is ...also stable at this temperature and can bind to the primers.  Extension- The temperature is then raised slightly..., or for generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction...What is polymerase chain reaction? If you have ever worked in a molecular biology laboratory you have...

...Page Read Other Posts in the Transferable Skills Guide Developing Transferable Skills During Science Training...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Collaborating with others outside... Addgene as a nonprofit organization is very considerate and thoughtful about its limited resources. Rather... will often look for partnerships where we can leverage another organization’s expertise to our mutual... other projects like it going, someone (likely several people at Addgene) had to take the initiative to...

...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: managing a team. You’ve just been... transferable skills...

...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this... highlighting that researchers do learn MANY transferable skills while working in the lab and by offering...where you might need some help. Today in our transferable skills guide: Time Mangement for Scientists.... Don’t be afraid to accomplish some quick but moderately useful tasks at the expense of time spent on ...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough...achieve these goals. I have far too many projects to operate this way now. Talking about “planning” is easy,... arises.Why do I write things down? It’s more viscerally appealing for me to physically cross things out...