We narrowed to 672 results for: FIE

1. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   ...Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid Collections! Join the Addgene...

2. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   ...Bramson, author “Coping with Difficult People,” identified seven types of difficult people: Hostile-aggressives...

3. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   ... indicate their presence. They are genetically modified organisms, and GMOs have a bad reputation. The...

4. AAV Titration by qPCR Using SYBR Green Technology

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   Protocol

   ...results when the plasmid is linearized. Treat the purified AAV samples with DNase I to eliminate any contaminating...

5. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ...have additional questions. If you or your lab identifies a potential collaborator from the meeting, reach...

6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ... guide and the DNA target. CFD scores are then modified (“transformed”) such that weak interactions (e.g...

7. 10 Basic tips for mammalian cell culture

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   Blog Post

   ...working with so that cross-contamination can be identified. Even if some mammalian cells lines have similar...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...more resources that may be more tailored to your field of study. 5 great resources to help get your fly...

9. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   ...proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported in vesicles ...

10. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post

    ...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...

11. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

    ...The concept is simple: deliver a template with a modified version of the targeted sequence to the cell which...

12. Hot Plasmids: Summer 2025

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    Blog Post

    ...fibronectin intrabody generated with mRNA display. Modified from Rivera & Huang, et al. (2025) under CC BY-NC-ND...

13. Hot Plasmids: Winter 2025

    Type

    Blog Post

    ...measured pixel and the soma. The initiation site identified in panel (C) is indicated; red line is exponential...

14. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...containing GEVIs. Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism...

15. Pooled Library Amplifications

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    Blog Post

    ...be useful to run a restriction digest of your amplified library on a gel to ensure that your conditions...

16. Plasmids 101: Degron Tags

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    Blog Post

    ...Degrons are destabilizing domains which will be identified by various enzymes and sent to the proteasome...

17. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...always effective. This is why recent work in the field of cancer research has focused on picking apart ...

18. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent...

19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ...treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins...

20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...early 2000s, a handful of chromoproteins were identified in sea anemone (Chan et al., 2006). Why do these...