We narrowed to 771 results for: LIS

1. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   ...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...

2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   ...precise determination of brightness in cells, under realistic experimental conditions. By determining the brightness...

3. Addgene’s Viral Service - Why Virus? Why Now?

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   ...Wyss Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral...

4. Viral Vectors 101: Viral Vector Elements

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   ... other relevant viral genes (including the ones listed previously). E3/E1B suppresses both apoptosis and...

5. Viral Vectors 101: Viral Applications

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   ...Versatile Viral tool for Gene Expression in Mammals Listen to Connie Cepko Discuss Gene Therapy with AAV ...

6. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...

8. Clear and Accessible Writing

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   ...alt text in the comments! We learned a lot from listening to others' alt text descriptions, and we’d love...

9. Developing a cold tolerant E. coli using specialized chaperones

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   ...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...

10. A Deep Dive into BioBuilder

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    .... (and Mexico in 2018). BioBuilder textbook, published by O’Reilly Media. BioBuilder's open-access curriculum...

11. Plasmids 101: Methylation and Restriction Enzymes

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    ...methylation site. The quick-reference table below lists 10 common enzymes that may be affected by methylation...

12. How to Keep a Lab Notebook for Bioinformatic Analyses

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    ...Find Protocols for Your Next Experiment Find Our List of CRISPR gRNA Design Tools ...

13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

14. Plasmids 101: Blue-white Screening

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    ...Cloning Technique for You Browse Addgene's curated list of Bacterial Expression Systems Find Empty Backbones...

15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...

16. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ... now. Enter the SAM pooled library In a paper published in Nature in December, Konermann and her colleagues...

17. 3 Challenges in Plant Synthetic Biology

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    ...biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work has also...

18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...visionary, a science-minded individual, and a specialist. Notably, the team must have an upfront conversation...

19. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ...have good ideas for who you should connect with. Listen to our podcast interview with Harvard Medical School...

20. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    ...to examine the effect of a gene on cholesterol metabolism. The TALEN kit is quite useful in generating ...