We narrowed to 840 results for: Met

...cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...One common biosensor class is designed to monitor metal ions. Within these ions, calcium is by far one of...

...deliver these systems virally instead of by another method? Viral delivery is attractive due to the ease of...viral vectors instead of a traditional transfection method. This is because viral systems have a high infection...

...cell (National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies, 1999). This...jbc.m116.736942  National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. Monoclonal ...

...with other scientists who maybe hadn’t used this method. I enjoyed writing the first post so I signed up... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...

...Alternatively, you can simply make a note in your methods section about the edit that was temporarily used...a lot of space and don’t tell you why you did something or what the result was, so try not to do this....

... from a polycistronic transcript. Although this method shortens construct size, it requires coexpression... be processed, a distinct advantage over other methods that favor certain nucleotides at the 5’ end. ...

...al, 2017) and cannot be described by a single parameter. Therefore, it is necessary to know how the fluorescence... (iii) attached to a substrate. These in vitro methods allow for a well-controlled environment, but they...

...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...

... a month or so of meetings, I began to notice something: even though our scientists and engineers were...likely will need to write protocols, SOPs, and methods sections. The next time you write or read one of...

...individuals with similar ideas to actually do something about it. Building a diverse team with different... industry partners, not the other way around. Sometimes, due to misconceptions and stereotypes, solving...

...test out lots of combinations, to end up finding something surprisingly useful. To apply these principles...requiring stable covalent reactions, other required parameters of the coupling system include: Wide fusion-tolerance...

...bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV....a large intervening deletion. Not only can this method precisely program large genomic deletions of up...

...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks."Molecular...

...actually don’t have to worry about and that is something to recognize! Distributing plasmids, viral service...before it was cool or a necessary result of the #MeToo movement. We are carefully attentive to genuine ...

...are no explicit instructions on what to do when something fails. While we’re sure our techs could figure...the contents of a tube with the tube itself. If something happens to the worm that requires a change of ...

...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

... elucidate cellular structures. Overexpression methods flood the cell with protein, which can both interfere...with BioRender.com. A key feature of our methodology is the use of a donor plasmid that contains long...

...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...on-target base editing functionality.  The delivery method of base editors plays a role for off-target editing...

...target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short vs. long ncRNAs The ...transcription of most of the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective...